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验证数据展示

  • WB analysis of HepG2 using 10089-2-AP (same clone as 10089-2-PBS)

    WB analysis of HepG2 using 10089-2-AP (same clone as 10089-2-PBS)

    WB result of COPS8/COP9 antibody (10089-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-COPS8/COP9 transfected HepG2 cells. This data was developed using the same antibody clone with 10089-2-PBS in a different storage buffer formulation.

  • WB analysis using 10089-2-AP (same clone as 10089-2-PBS)

    WB analysis using 10089-2-AP (same clone as 10089-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10089-2-AP (COPS8/COP9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10089-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10089-2-AP (same clone as 10089-2-PBS)

    IHC staining of human colon cancer using 10089-2-AP (same clone as 10089-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10089-2-AP (COPS8/COP9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10089-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10089-2-AP (same clone as 10089-2-PBS)

    IHC staining of human colon cancer using 10089-2-AP (same clone as 10089-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10089-2-AP (COPS8/COP9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10089-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 10089-2-AP (same clone as 10089-2-PBS)

    IP experiment of mouse brain using 10089-2-AP (same clone as 10089-2-PBS)

    IP result of anti-COPS8/COP9 (IP:10089-2-AP, 4ug; Detection:10089-2-AP 1:500) with mouse brain tissue lysate 3200 ug. This data was developed using the same antibody clone with 10089-2-PBS in a different storage buffer formulation.

COPS8/COP9 Polyclonal antibody, PBS Only

COPS8/COP9 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 10089-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

COP9, COPS8, CSN8, hCOP9, JAB1-containing signalosome subunit 8

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10089-2-PBS targets COPS8/COP9 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0143

Product name: Recombinant human COPS8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 14-194 aa of BC003090

Sequence: KKLLDQCENQELEAPGGIATPPVYGQLLALYLLHNDMNNARYLWKRIPPAIKSANSELGGIWSVGQRIWQRDFPGIYTTINAHQWSETVQPIMEALRDATRRRAFALVSQAYTSIIADDFAAFVGLPVEEAVKGILEQGWQADSTTRMVLPRKPVAGALDVSFNKFIPLSEPAPVPPIPNE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 COP9 constitutive photomorphogenic homolog subunit 8 (Arabidopsis)
别名 COP9, COPS8, CSN8, hCOP9, JAB1-containing signalosome subunit 8
计算分子量 23 kDa
观测分子量 23 kDa
GenBank蛋白编号BC003090
基因名称 COPS8
Gene ID (NCBI) 10920
RRIDAB_2081903
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ99627
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COPS8 is one of the eight subunits of COP9 signalosome, a highly conserved protein complex that functions as an important regulator in multiple signaling pathways. The structure and function of COP9 signalosome is similar to that of the 19S regulatory particle of 26S proteasome. COP9 signalosome has been shown to interact with SCF-type E3 ubiquitin ligases and act as a positive regulator of E3 ubiquitin ligases.

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