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验证数据展示

  • WB analysis using 16628-1-AP

    WB analysis using 16628-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16628-1-AP (COPS7A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 16628-1-AP

    IP experiment of HeLa using 16628-1-AP

    IP result of anti-COPS7A (IP:16628-1-AP, 4ug; Detection:16628-1-AP 1:5000) with HeLa cells lysate 1600 ug.

  • IHC staining of mouse brain using 16628-1-AP

    IHC staining of mouse brain using 16628-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16628-1-AP (COPS7A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MCF-7 using 16628-1-AP

    IF Staining of MCF-7 using 16628-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using COPS7A antibody (16628-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

COPS7A Polyclonal antibody

COPS7A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 16628-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

COPS, CSN7A, Dermal papilla-derived protein 10, DERP10, JAB1-containing signalosome subunit 7a

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRAW 264.7 cells, mouse brain tissue, PC-12 cells, HeLa cells, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16628-1-AP targets COPS7A in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9958

Product name: Recombinant human COPS7A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-275 aa of BC011789

Sequence: MSAEVKVTGQNQEQFLLLAKSAKGAALATLIHQVLEAPGVYVFGELLDMPNVRELAESDFASTFRLLTVFAYGTYADYLAEARNLPPLTEAQKNKLRHLSVVTLAAKVKCIPYAVLLEALALRNVRQLEDLVIEAVYADVLRGSLDQRNQRLEVDYSIGRDIQRQDLSAIARTLQEWCVGCEVVLSGIEEQVSRANQHKEQQLGLKQQIESEVANLKKTIKVTTAAAAAATSQDPEQHLTELREPAPGTNQRQPSKKASKGKGLRGSAKIWSKSN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 COP9 constitutive photomorphogenic homolog subunit 7A (Arabidopsis)
别名 COPS, CSN7A, Dermal papilla-derived protein 10, DERP10, JAB1-containing signalosome subunit 7a
计算分子量 275 aa, 30 kDa
观测分子量 30 kDa
GenBank蛋白编号BC011789
基因名称 COPS7A
Gene ID (NCBI) 50813
RRIDAB_2081787
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UBW8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPS7A is component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. COPS7A was consistently expressed in oligodendrocyte lineage cells, and was down-regulated during the peak period of myelination. COPS7A associated physically with Olig1, and it might thus serve as a novel negative regulator in oligodendrocytes differentiation.

实验方案

Product Specific Protocols
IF protocol for COPS7A antibody 16628-1-APDownload protocol
IHC protocol for COPS7A antibody 16628-1-APDownload protocol
IP protocol for COPS7A antibody 16628-1-APDownload protocol
WB protocol for COPS7A antibody 16628-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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