验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, 3T3-L1 cells, pig lymph node tissue |
| Positive IHC detected in | human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, COLO 320 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
10256-1-AP targets COPS6 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, monkey samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat, pig, monkey |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag0331 Product name: Recombinant human COPS6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 40-292 aa of BC002520 Sequence: SVALHPLVILNISDHWIRMRSQEGRPVQVIGALIGKQEGRNIEVMNSFELLSHTVEEKIIIDKEYYYTKEEQFKQVFKELEFLGWYTTGGPPDPSDIHVHKQVCEIIESPLFLKLNPMTKHTDLPVSVFESVIDIINGEATMLFAELTYTLATEEAERIGVDHVARMTATGSGENSTVAEHLIAQHSAIKMLHSRVKLILEYVKASEAGEVPFNHEILREAYALCHCLPVLSTDKFKTDFYDQCNDVGLMAYL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis) |
| 别名 | CSN6, Signalosome subunit 6, SGN6, MOV34 34KD, JAB1-containing signalosome subunit 6 |
| 计算分子量 | 327 aa, 36 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC002520 |
| 基因名称 | COPS6 |
| Gene ID (NCBI) | 10980 |
| RRID | AB_2034908 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q7L5N1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The COPS6 protein, also named mov34, is a 34 kDa subunit of COP9 signalosome, a highly conserved eight-subunit protein complex that functions as an important regulator in multiple signaling pathways (PMID: 38308184). This protein belongs to translation initiation factor 3 (eIF3) superfamily. It is involved in the regulation of cell cycle and acts as the regulatory subunit of the 26S proteasome.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for COPS6 antibody 10256-1-AP | Download protocol |
| IHC protocol for COPS6 antibody 10256-1-AP | Download protocol |
| WB protocol for COPS6 antibody 10256-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
