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验证数据展示

  • WB analysis using 30494-1-AP

    WB analysis using 30494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30494-1-AP (COPS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 30494-1-AP

    WB analysis of HeLa using 30494-1-AP

    WB result of COPS3 antibody (30494-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COPS3 transfected HeLa cells.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30494-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30494-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30494-1-AP (COPS3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COPS3 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 30494-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30494-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30494-1-AP (COPS3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COPS3 antibody included
  • Featured Product
  • KD/KO验证

COPS3 Polyclonal antibody

COPS3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30494-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

JAB1-containing signalosome subunit 3, COP 9 signalosome complex subunit 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy COPS3 Ready-To-Use IHC Kit. COPS3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30494-1-AP targets COPS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag32825

Product name: Recombinant human COPS3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 270-423 aa of BC001891

Sequence: NNPSELRNLVNKHSETFTRDNNMGLVKQCLSSLYKKNIQRLTKTFLTLSLQDMASRVQLSGPQEAEKYVLHMIEDGEIFASINQKDGMVSFHDNPEKYNNPAMLHNIDQEMLKCIELDERLKAMDQEITVNPQFVQKSMGSQEDDSGNKPSSYS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
别名 JAB1-containing signalosome subunit 3, COP 9 signalosome complex subunit 3
计算分子量 48 kDa
观测分子量40 kDa
GenBank蛋白编号BC001891
基因名称 COPS3
Gene ID (NCBI) 8533
RRIDAB_3086336
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNS2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPS3 possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

实验方案

Product Specific Protocols
WB protocol for COPS3 antibody 30494-1-APDownload protocol
IHC protocol for COPS3 antibody 30494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols