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验证数据展示

  • WB analysis using 30494-1-AP

    WB analysis using 30494-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30494-1-AP (COPS3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 30494-1-AP

    WB analysis of HeLa using 30494-1-AP

    WB result of COPS3 antibody (30494-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COPS3 transfected HeLa cells.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30494-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30494-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30494-1-AP (COPS3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COPS3 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 30494-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30494-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30494-1-AP (COPS3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COPS3 antibody included
  • Featured Product
  • KD/KO验证

COPS3 Polyclonal antibody

COPS3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30494-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

JAB1-containing signalosome subunit 3, COP 9 signalosome complex subunit 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy COPS3 Ready-To-Use IHC Kit. COPS3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30494-1-AP targets COPS3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 COPS3 fusion protein Ag32825 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 COP9 constitutive photomorphogenic homolog subunit 3 (Arabidopsis)
别名 JAB1-containing signalosome subunit 3, COP 9 signalosome complex subunit 3
计算分子量 48 kDa
观测分子量40 kDa
GenBank蛋白编号BC001891
基因名称 COPS3
Gene ID (NCBI) 8533
RRIDAB_3086336
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UNS2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COPS3 possesses kinase activity that phosphorylates regulators involved in signal transduction. It phosphorylates I kappa-Balpha, p105, and c-Jun. It acts as a docking site for complex-mediated phosphorylation. The gene is located within the Smith-Magenis syndrome region on chromosome 17.

实验方案

Product Specific Protocols
WB protocol for COPS3 antibody 30494-1-APDownload protocol
IHC protocol for COPS3 antibody 30494-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols