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验证数据展示

  • WB analysis using 27469-1-AP

    WB analysis using 27469-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27469-1-AP (COPB1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 27469-1-AP

    IHC staining of human stomach using 27469-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 27469-1-AP (COPB1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COPB1 antibody included

COPB1 Polyclonal antibody

COPB1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27469-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

COPB, Coatomer subunit beta, Beta-COP, Beta-coat protein, Beta COP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, mouse brain tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy COPB1 Ready-To-Use IHC Kit. COPB1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27469-1-AP targets COPB1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag26518

Product name: Recombinant human COPB1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 288-544 aa of BC037280

Sequence: LIIKESDNNVKLIVLDRLIELKEHPAHERVLQDLVMDILRVLSTPDLEVRKKTLQLALDLVSSRNVEELVIVLKKEVIKTNNVSEHEDTDKYRQLLVRTLHSCSVRFPDMAANVIPVLMEFLSDNNEAAAADVLEFVREAIQRFDNLRMLIVEKMLEVFHAIKSVKIYRGALWILGEYCSTKEDIQSVMTEIRRSLGEIPIVESEIKKEAGELKPEEEITVGPVQKLVTEMGTYATQSALSSSRPTKKEEDRPPLRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coatomer protein complex, subunit beta 1
别名 COPB, Coatomer subunit beta, Beta-COP, Beta-coat protein, Beta COP
计算分子量 107 kDa
观测分子量 95-100 kDa
GenBank蛋白编号BC037280
基因名称 COPB1
Gene ID (NCBI) 1315
RRIDAB_2880880
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP53618
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COPB1 antibody 27469-1-APDownload protocol
IHC protocol for COPB1 antibody 27469-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols