验证数据展示
经过测试的应用
| Positive WB detected in | HCT 116 cells |
| Positive IP detected in | HCT 116 cells, HeLa cells |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28395-1-AP targets COP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag28163 Product name: Recombinant human COP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 274-493 aa of BC039723 Sequence: EMSGLYSPVSEDSTVPQFEAPSPSHSSIIDSTEYSQPPGFSGSSQTKKQPWYNSTLASRRKRLTAHFEDLEQCYFSTRMSRISDDSRTASQLDEFQECLSKFTRYNSVRPLATLSYASDLYNGSSIVSSIEFDRDCDYFAIAGVTKKIKVYEYDTVIQDAVDIHYPENEMTCNSKISCISWSSYHKNLLASSDYEGTVILWDGFTGQRSKVYQEHEKRCW 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ring finger and WD repeat domain 2 |
| 别名 | RFWD2, E3 ubiquitin-protein ligase COP1, EC:2.3.2.27, hCOP1, RING finger protein 200 |
| 计算分子量 | 731 aa, 80 kDa |
| 观测分子量 | 80-90 kDa |
| GenBank蛋白编号 | BC039723 |
| 基因名称 | COP1 |
| Gene ID (NCBI) | 64326 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8NHY2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COP1 (constitutive photomorphogenic 1) is also named Ring finger and WD domain 2 (RFWD2), RING finger protein 200 (RNF20). COP1 is an E3 ubiquitin ligase that modifies specific target proteins, priming their degradation via the ubiquitin proteasome system (PMID: 27335464). COP1 (also known as RFWD2) is an important suppressor of pathogenic c/EBPβ-dependent gene expression programs in microglia (PMID: 32795415). COP1 is as a tumour suppressor that negatively regulates ETV1, ETV4 and ETV5. The ubiquitin ligase COP1 is as a tumour suppressor whose downregulation promotes prostatic epithelial cell proliferation and tumorigenesis (PMID: 21572435). COP1 has been identified as a novel and critical negative regulator of the tumor suppressor protein p53, and it influences p53-dependent transcription, apoptosis, cell cycle arrest and DNA repair (PMID: 16931761, PMID: 15103385). Recently, COP1 has been found to be overexpressed in human hepatocellular carcinoma, breast cancer and ovarian adenocarcinomas (PMID: 15492238, PMID: 20959491).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for COP1 antibody 28395-1-AP | Download protocol |
| IP protocol for COP1 antibody 28395-1-AP | Download protocol |
| WB protocol for COP1 antibody 28395-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
