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验证数据展示

  • WB analysis using 84664-4-RR

    WB analysis using 84664-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84664-4-RR (COMMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat testis using 84664-4-RR

    IHC staining of rat testis using 84664-4-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84664-4-RR (COMMD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat testis using 84664-4-RR

    IHC staining of rat testis using 84664-4-RR

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84664-4-RR (COMMD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84664-4-RR

    Affinity and Kinetic Characterization of 84664-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84664-4-RR against Human COMMD4 were performed. The affinity constant is below 1 pM.

COMMD4 Recombinant antibody

COMMD4 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84664-4-RR

产品说明书

CloneNo. 242175F4

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

COMMD 4, COMM domain containing 4, 242175F4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, MCF-7 cells, HeLa cells, HEK-293T cells, U2OS cells
Positive IHC detected inrat testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84664-4-RR targets COMMD4 in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, rat
免疫原 COMMD4 fusion protein Ag34904 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 COMM domain containing 4
别名 COMMD 4, COMM domain containing 4, 242175F4
观测分子量25 kDa
GenBank蛋白编号BC068998
基因名称 COMMD4
Gene ID (NCBI) 54939
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0A8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COMMD4 is a protein-coding gene belonging to the COMMD family and is highly expressed in non-small cell lung cancer (NSCLC) and hepatocellular carcinoma (HCC) (PMID: 36249824). It has 3 isoforms and has been shown to control NF-κB activity and regulate the activity of cullin-RING E3 ubiquitin ligases (PMID: 32439936).

实验方案

Product Specific Protocols
WB protocol for COMMD4 antibody 84664-4-RRDownload protocol
IHC protocol for COMMD4 antibody 84664-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols