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验证数据展示

  • WB analysis using 84664-4-RR (same clone as 84664-4-PBS)

    WB analysis using 84664-4-RR (same clone as 84664-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84664-4-RR (COMMD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84664-4-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 84664-4-RR (same clone as 84664-4-PBS)

    IHC staining of rat testis using 84664-4-RR (same clone as 84664-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84664-4-RR (COMMD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84664-4-PBS in a different storage buffer formulation.

  • IHC staining of rat testis using 84664-4-RR (same clone as 84664-4-PBS)

    IHC staining of rat testis using 84664-4-RR (same clone as 84664-4-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 84664-4-RR (COMMD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84664-4-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 84664-4-RR (same clone as 84664-4-PBS)

    WB analysis of MCF-7 using 84664-4-RR (same clone as 84664-4-PBS)

    WB result of COMMD4 antibody (84664-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-COMMD4 transfected MCF-7 cells. This data was developed using the same antibody clone with 84664-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84664-4-RR

    Affinity and Kinetic Characterization of 84664-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84664-4-RR against Human COMMD4 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

COMMD4 Recombinant monoclonal antibody, PBS Only

COMMD4 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 84664-4-PBS

产品说明书

CloneNo. 242175F4

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, Indirect ELISA

别名

242175F4, COMM domain containing 4, COMMD 4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84664-4-PBS targets COMMD4 in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

CatNo: Ag34904

Product name: Recombinant human COMMD4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-199 aa of BC068998

Sequence: MRFRFCGDLDCPDWVLAEISTLAKMSSVKLRLLCSQVLKELLGQGIDYEKILKLTADAKFESGDVKATVAVLSFILSSAAKHSVDGESLSSELQQLGLPKEHAASLCRCYEEKQSPLQKHLRVCSLRMNRLAGVGWRVDYTLSSSLLQSVEEPMVHLRLEVAAAPGTPAQPVAMSLSADKFQVLLAELKQAQTLMSSLG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 COMM domain containing 4
别名 242175F4, COMM domain containing 4, COMMD 4
观测分子量25 kDa
GenBank蛋白编号BC068998
基因名称 COMMD4
Gene ID (NCBI) 54939
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H0A8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

COMMD4 is a protein-coding gene belonging to the COMMD family and is highly expressed in non-small cell lung cancer (NSCLC) and hepatocellular carcinoma (HCC) (PMID: 36249824). It has 3 isoforms and has been shown to control NF-κB activity and regulate the activity of cullin-RING E3 ubiquitin ligases (PMID: 32439936).

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