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验证数据展示

  • WB analysis using 34055-1-AP

    WB analysis using 34055-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 34055-1-AP (COLEC12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis using 34055-1-AP

    WB analysis using 34055-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 34055-1-AP (COLEC12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

COLEC12 Polyclonal antibody

COLEC12 Polyclonal Antibody for WB, ELISA
Cat No. 34055-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

CL P1, CLP1, CL-P1, Collectin 12, Collectin placenta protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

34055-1-AP targets COLEC12 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag39696

Product name: Recombinant human COLEC12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 140-433 aa of BC060789

Sequence: QASGDALVDRQSQLKETLENNSFLITTVNKTLQAYNGYVTNLQQDTSVLQGNLQNQMYSHNVVIMNLNNLNLTQVQQRNLITNLQRSVDDTSQAIQRIKNDFQNLQQVFLQAKKDTDWLKEKVQSLQTLAANNSALAKANNDTLEDMNSQLNSFTGQMENITTISQANEQNLKDLQDLHKDAENRTAIKFNQLEERFQLFETDIVNIISNISYTAHHLRTLTSNLNEVRTTCTDTLTKHTDDLTSLNNTLANIRLDSVSLRMQQDLMRSRLDTEVANLSVIMEEMKLVDSKHGQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collectin sub-family member 12
别名 CL P1, CLP1, CL-P1, Collectin 12, Collectin placenta protein 1
计算分子量 82 kDa
观测分子量140 kDa, 90 kDa, 70 kDa,
GenBank蛋白编号BC060789
基因名称 COLEC12
Gene ID (NCBI) 81035
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ5KU26
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Colonin-12 (COLEC12), also known as colonin subfamily member 12 or CL-P1, is a pattern recognition receptor belonging to the colonin family. As a scavenger receptor, it plays a role in innate immunity, recognizing pathogen-associated molecular patterns (PAMPs) and damaged self-components. COLEC12 plays a role in pathogen clearance, apoptotic cell recognition, and the regulation of inflammatory responses. COLEC12 is also associated with cardiovascular disease and host defense mechanisms. (PMID: 10224141; 27377710;11564734; 24058540)

实验方案

Product Specific Protocols
WB protocol for COLEC12 antibody 34055-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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