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验证数据展示

  • WB analysis using 68320-1-Ig (same clone as 68320-1-PBS)

    WB analysis using 68320-1-Ig (same clone as 68320-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    WB analysis of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • WB analysis of human rectum using 68320-1-Ig (same clone as 68320-1-PBS)

    WB analysis of human rectum using 68320-1-Ig (same clone as 68320-1-PBS)

    human rectum tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • WB analysis of rabbit large intestine using 68320-1-Ig (same clone as 68320-1-PBS)

    WB analysis of rabbit large intestine using 68320-1-Ig (same clone as 68320-1-PBS)

    rabbit large intestine tissue were subjected to SDS PAGE followed by western blot with 68320-1-Ig (COL3A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of human ovary tumor using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of mouse skin using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of mouse skin using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of human placenta using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    IHC staining of human hepatocirrhosis using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68320-1-Ig (COL3A1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with COL3A1 antibody included
  • IF Staining of human colon cancer using 68320-1-Ig (same clone as 68320-1-PBS)

    IF Staining of human colon cancer using 68320-1-Ig (same clone as 68320-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using Collagen Type III antibody (68320-1-Ig, Clone: 3B1F3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68320-1-PBS in a different storage buffer formulation.

Collagen Type III Monoclonal antibody, PBS Only

Collagen Type III Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 68320-1-PBS

产品说明书

CloneNo. 3B1F3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF-P, Indirect ELISA

别名

COL3, COL3A1, 3B1F3, Collagen alpha 1(III) chain, Collagen alpha-1(III) chain

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

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Planning an IHC experiment? We recommend our IHCeasy COL3A1 Ready-To-Use IHC Kit. COL3A1 primary antibody included.

产品信息

68320-1-PBS targets Collagen Type III in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原

CatNo: Ag4229

Product name: Recombinant human COL3A1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1150-1466 aa of BC028178

Sequence: DGTSGHPGPIGPPGPRGNRGERGSEGSPGHPGQPGPPGPPGAPGPCCGGVGAAAIAGIGGEKAGGFAPYYGDEPMDFKINTDEIMTSLKSVNGQIESLISPDGSRKNPARNCRDLKFCHPELKSGEYWVDPNQGCKLDAIKVFCNMETGETCISANPLNVPRKHWWTDSSAEKKHVWFGESMDGGFQFSYGNPELPEDVLDVQLAFLRLLSSRASQNITYHCKNSIAYMDQASGNVKKALKLMGSNEGEFKAEGNSKFTYTVLEDGCTKHTGEWSKTVFEYRTRKAVRLPIVDIAPYDIGGPDQEFGVDVGPVCFL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 collagen, type III, alpha 1
别名 COL3, COL3A1, 3B1F3, Collagen alpha 1(III) chain, Collagen alpha-1(III) chain
计算分子量 1466 aa, 139 kDa
观测分子量130 kDa
GenBank蛋白编号BC028178
基因名称 COL3A1
Gene ID (NCBI) 1281
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP02461
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Type III collagen is a fibrillar-forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome type IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341).

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