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Collagen Type III (N-terminal) Polyclonal antibody
Collagen Type III (N-terminal) Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (6)
Applications
WB, IHC, IF-P, IF-Fro, IP, ELISA and More (1)
Conjugate
Unconjugated
467
验证数据展示
经过测试的应用
Positive WB detected in | mouse skin tissue, rat skin tissue |
Positive IP detected in | mouse skin tissue |
Positive IHC detected in | human hepatocirrhosis tissue, human pancreas cancer tissue, mouse liver tissue, mouse kidney tissue, human colon tissue, human skin cancer tissue, mouse heart tissue, mouse colon tissue, human skin tissue, human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human colon tissue |
Positive IF-Fro detected in | mouse colon tissue |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:300-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
22734-1-AP targets Collagen Type III (N-terminal) in WB, IHC, IF-P, IF-Fro, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF-P, IF-Fro, IP, ELISA Application Description |
Cited Applications | WB, IHC, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, rabbit, canine, chicken, bovine, hamster |
Immunogen | Collagen Type III (N-terminal) fusion protein Ag18658 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | collagen, type III, alpha 1 |
Synonyms | COL3A1, COL3, collagen, type III, alpha 1, Collagen Type III, Collagen alpha-1(III) chain |
Calculated Molecular Weight | 1466 aa, 139 kDa |
Observed Molecular Weight | 140-180 kDa |
GenBank Accession Number | BC028178 |
Gene Symbol | COL3A1 |
Gene ID (NCBI) | 1281 |
RRID | AB_2879158 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P02461 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Collagen Type III (N-terminal) antibody 22734-1-AP | Download protocol |
IHC protocol for Collagen Type III (N-terminal) antibody 22734-1-AP | Download protocol |
IF protocol for Collagen Type III (N-terminal) antibody 22734-1-AP | Download protocol |
IP protocol for Collagen Type III (N-terminal) antibody 22734-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
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