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Collagen Type XXVII Polyclonal antibody

Collagen Type XXVII Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15673-1-AP

Print datasheet

Synonyms

COL27A1, Collagen alpha 1(XXVII) chain, Collagen Type XXVII, collagen, type XXVII, alpha 1, KIAA1870

验证数据展示

  • WB analysis using 15673-1-AP

    WB analysis using 15673-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15673-1-AP (Collagen Type XXVII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 15673-1-AP

    IHC staining of human placenta using 15673-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15673-1-AP (Collagen Type XXVII antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, mouse brain tissue, mouse heart tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15673-1-AP targets Collagen Type XXVII in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse
Immunogen Collagen Type XXVII fusion protein Ag8140 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name collagen, type XXVII, alpha 1
Synonyms COL27A1, Collagen alpha 1(XXVII) chain, Collagen Type XXVII, collagen, type XXVII, alpha 1, KIAA1870
Calculated Molecular Weight 1860 aa, 87 kDa
Observed Molecular Weight68-73 kDa
GenBank Accession NumberBC007696
Gene Symbol COL27A1
Gene ID (NCBI) 85301
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IZC6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Collagen Type XXVII, also named COL27A1, it is a member of the collagen fibrillar gene family . It is located in the extrcellular matrix. The calculated molecular weight of COL27A1 is 186 kDa. This antibody can recognize the 73 kDa isoform of target. The high level of Col27a1 expression in mouse cartilage suggested the possibility that Col27a1 may be regulated in cartilage by SOX9, as are Col2a1, Col11a2, Col9a1, and Col9a2 (PMID: 15922909). Expression analyses of the mouse col27a1 gene demonstrated high expression in cartilage, the eye and ear, but also in lung and colon. It is likely that the major protein product of COL27A1, proa1(XXVII), is a component of the extracellular matrices of cartilage and these other tissues. Study of this collagen should yield insights into normal chondrogenesis, and provide clues to the pathogenesis of some chondrodysplasias and disorders of other tissues in which this gene is expressed (PMID: 12714037).

实验方案

Product Specific Protocols
WB protocol for Collagen Type XXVII antibody 15673-1-APDownload protocol
IHC protocol for Collagen Type XXVII antibody 15673-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols