验证数据展示
经过测试的应用
| Positive WB detected in | rat skin tissue, pig skin tissue, mouse tail tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66761-1-Ig targets Collagen Type I in WB, IHC, IF, ELISA, IHC-IF applications and shows reactivity with Human, pig, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IHC-IF |
| 经测试反应性 | Human, pig, mouse, rat |
| 文献引用反应性 | human, mouse, rat, rabbit, bovine |
| 免疫原 | Collagen Type I fusion protein Ag6281 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | collagen, type I, alpha 2 |
| 别名 | Alpha 2 type I collagen, COL1A2, Collagen alpha 2(I) chain, Collagen Type I, collagen, type I, alpha 2, OI4 |
| 计算分子量 | 1366 aa, 130 kDa |
| 观测分子量 | 140 kDa, 70-80 kDa |
| GenBank蛋白编号 | BC054498 |
| 基因名称 | COL1A2 |
| Gene ID (NCBI) | 1278 |
| RRID | AB_2882107 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P08123 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Collagen Type I antibody 66761-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Acta Pharm Sin B Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1. | ||
Adv Healthc Mater Metformin Carbon Dots for Promoting Periodontal Bone Regeneration via Activation of ERK/AMPK Pathway. | ||
Clin Transl Med Histone methyltransferase KMT2A promotes pulmonary fibrogenesis via targeting pro-fibrotic factor PU.1 in fibroblasts | ||
Cell Mol Life Sci Dysregulated cholesterol uptake and efflux of bone marrow-derived α-SMA+ macrophages contribute to atherosclerotic plaque formation | ||
Phytomedicine β-elemene alleviates hyperglycemia-induced cardiac inflammation and remodeling by inhibiting the JAK/STAT3-NF-κB pathway | ||
Oxid Med Cell Longev Eugenol-Preconditioned Mesenchymal Stem Cell-Derived Extracellular Vesicles Promote Antioxidant Capacity of Tendon Stem Cells In Vitro and In Vivo. |
