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验证数据展示

  • WB analysis using 19801-1-AP

    WB analysis using 19801-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 19801-1-AP (Collagen Type XIII antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Collagen Type XIII Polyclonal antibody

Collagen Type XIII Polyclonal Antibody for WB, ELISA
Cat No. 19801-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

COL13, COL13A1, Collagen alpha 1(XIII) chain, Collagen alpha-1(XIII) chain, COLXIIIA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHT-1080 cells, NIH/3T3 eclls

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19801-1-AP targets Collagen Type XIII in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原

fusion protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 collagen, type XIII, alpha 1
别名 COL13, COL13A1, Collagen alpha 1(XIII) chain, Collagen alpha-1(XIII) chain, COLXIIIA1
计算分子量 70 kDa
观测分子量62-70 kDa, 85-100 kDa
GenBank蛋白编号NM_001130103
基因名称 COL13A1
Gene ID (NCBI) 1305
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5TAT6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COL13A1 is involved in cell-matrix and cell-cell adhesion interactions that are required for normal development. COL13A1 may participate in the linkage between muscle fiber and basement membrane. COL13A1 may play a role in endochondral ossification of bone and branching morphogenesis of lung. COL13A1 can bind heparin. The antibody can recognize all the isoforms except isoform9 of COL13A1. This antibody is specific to COL13A1. It is predicted molecular weight is 62-70 kDa, and it is also reported as 85-95 kDa (PMID:11223332).

实验方案

Product Specific Protocols
WB protocol for Collagen Type XIII antibody 19801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols