验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, MCF-7 cells, Raji cells |
| Positive IHC detected in | human ovary cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
12661-1-AP targets COG8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag3341 Product name: Recombinant human COG8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 394-612 aa of BC017492 Sequence: MNSYMLISAPAILGTSNMPAAVPATQPGTLQPPMVLLDFPPLACFLNNILVAFNDLRLCCPVALAQDVTGALEDALAKVTKIILAFHRAEEAAFSSGEQELFVQFCTVFLEDLVPYLNRCLQVLFPPAQIAQTLGIPPTQLSKYGNLGHVNIGAIQEPLAFILPKRETLFTLDDQALGPELTAPAPEPPAEEPRLEPAGPACPEGGRAETQAEPPSVGP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | component of oligomeric golgi complex 8 |
| 别名 | COG complex subunit 8, DOR1 |
| 计算分子量 | 612 aa, 68 kDa |
| 观测分子量 | 68 kDa |
| GenBank蛋白编号 | BC017492 |
| 基因名称 | COG8 |
| Gene ID (NCBI) | 84342 |
| RRID | AB_2244993 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96MW5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The conserved oligomeric Golgi (COG) complex is an eight subunit (COG1-8) peripheral Golgi membrane hetero-oligomeric protein complex. The COG complex is organized into lobes A (COG2-4) and B (COG5-7) with COG1 and COG8 bridging these lobes and is thought to play a critical role in vesicle tethering processes (PMID: 17331980). Cog8 localizes exclusively in the Golgi apparatus and is involved in the retrograde Golgi transport of resident proteins responsible for sugar chain (glycan) biosynthesis (PMID: 17220172).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for COG8 antibody 12661-1-AP | Download protocol |
| IHC protocol for COG8 antibody 12661-1-AP | Download protocol |
| WB protocol for COG8 antibody 12661-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
