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验证数据展示

  • WB analysis using 13552-1-AP

    WB analysis using 13552-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13552-1-AP (COG7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of Y79 using 13552-1-AP

    WB analysis of Y79 using 13552-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13552-1-AP (COG7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human cervical cancer using 13552-1-AP

    IHC staining of human cervical cancer using 13552-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13552-1-AP (COG7 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COG7 antibody included
  • IHC staining of human cervical cancer using 13552-1-AP

    IHC staining of human cervical cancer using 13552-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13552-1-AP (COG7 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COG7 antibody included

COG7 Polyclonal antibody

COG7 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13552-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

COG complex subunit 7, UNQ3082/PRO10013

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Y79 cells, HeLa cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy COG7 Ready-To-Use IHC Kit. COG7 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13552-1-AP targets COG7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4335

Product name: Recombinant human COG7 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 419-770 aa of BC037563

Sequence: CGLLSALKSLFAKYVSDFTSTLQSIRKKCKLDHIPPNSLFQEDWTAFQNSIRIIATCGELLRHCGDFEQQLANRILSTAGKYLSDSCSPRSLAGFQESILTDKKNSAKNPWQEYNYLQKDNPAEYASLMEILYTLKEKGSSNHNLLAAPRAALTRLNQQAHQLAFDSVFLRIKQQLLLISKMDSWNTAGIGETLTDELPAFSLTPLEYISNIGQYIMSLPLNLEPFVTQEDSALELALHAGKLPFPPEQGDELPELDNMADNWLGSIARATMQTYCDAILQIPELSPHSAKQLATDIDYLINVMDALGLQPSRTLQHIVTLLKTRPEDYRQVSKGLPRRLATTVATMRSVNY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 component of oligomeric golgi complex 7
别名 COG complex subunit 7, UNQ3082/PRO10013
计算分子量 770 aa, 86 kDa
观测分子量 86 kDa
GenBank蛋白编号BC037563
基因名称 COG7
Gene ID (NCBI) 91949
RRIDAB_2229608
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP83436
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COG7 (Component of Oligomeric Golgi Complex 7) is a critical protein-coding gene located on chromosome 16p12.2. By maintaining the structure and environment of the Golgi, COG7 is essential for the proper attachment of sugar chains (glycosylation) to proteins and lipids.

实验方案

Product Specific Protocols
IHC protocol for COG7 antibody 13552-1-APDownload protocol
WB protocol for COG7 antibody 13552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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