验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, LNCaP cells, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31380-1-AP targets COG5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag35394 Product name: Recombinant human COG5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of NM_181733.2 Sequence: MGWVGGRRRDSASPPGRSRSAADDINPAPANMEGGGGSVAVAGLGARGSGAAAATVRELLQDGCYSDFLNEDFDVKTYTSQSIHQAVIAEQLAKLAQGISQLDRELHLQVVARHEDLLAQATGIESLEGVLQMMQTRIGALQGAVDRIKAKIVEPYNKIVARTAQLARLQVACDLLRRIIRILNLSKRLQGQLQGGSREI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | component of oligomeric golgi complex 5 |
| 别名 | Golgi transport complex 1, GOLTC1, GTC90 |
| 计算分子量 | 93kDa，839aa |
| 观测分子量 | 87-93 kDa |
| GenBank蛋白编号 | NM_181733.2 |
| 基因名称 | COG5 |
| Gene ID (NCBI) | 10466 |
| RRID | AB_3742422 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9UP83 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
COG5 (Component Of Oligomeric Golgi Complex 5) is part of an eight-subunit Golgi-resident protein referred to as the conserved oligomeric golgi (COG) complex, which facilitates retrograde Golgi trafficking of vesicles that may include necessary glycosylation enzymes (PMID: 33277529). COG5 knockdown led to marked increases indicative of aberrant glycosylation (PMID: 25331899). The 92- and 87-kDa polypeptides could also be alternatively spliced isoforms of GTC-90 (PMID: 9792665)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COG5 antibody 31380-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
