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COG3 Polyclonal antibody

COG3 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11130-1-AP

Print datasheet

Synonyms

COG complex subunit 3, COG3, p94, SEC34

验证数据展示

  • WB analysis using 11130-1-AP

    WB analysis using 11130-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11130-1-AP (COG3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11130-1-AP

    IHC staining of human prostate cancer using 11130-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11130-1-AP (COG3 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with COG3 antibody included
  • IP experiment of HeLa using 11130-1-AP

    IP experiment of HeLa using 11130-1-AP

    IP result of anti-COG3 (IP:11130-1-AP, 4ug; Detection:11130-1-AP 1:500) with HeLa cells lysate 1200ug.

经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, mouse liver, rat liver
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy COG3 Ready-To-Use IHC Kit. COG3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11130-1-AP targets COG3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen COG3 fusion protein Ag1610 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name component of oligomeric golgi complex 3
Synonyms COG complex subunit 3, COG3, p94, SEC34
Calculated Molecular Weight 94 kDa
Observed Molecular Weight 94 kDa
GenBank Accession NumberBC038953
Gene Symbol COG3
Gene ID (NCBI) 83548
RRIDAB_2081402
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96JB2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for COG3 antibody 11130-1-APDownload protocol
IHC protocol for COG3 antibody 11130-1-APDownload protocol
IP protocol for COG3 antibody 11130-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human

Cancer Cell

Systematic Functional Annotation of Somatic Mutations in Cancer.

Authors - Patrick Kwok-Shing Ng
View Article
humanIP

Cancer Cell

A-to-I RNA Editing Contributes to Proteomic Diversity in Cancer.

Authors - Xinxin Peng
View Article
humanWB

Cancer Cell

The Genomic Landscape and Clinical Relevance of A-to-I RNA Editing in Human Cancers.

Authors - Leng Han
View Article
humanWB,IP

Cancer Immunol Res

PMID: 32265228

Authors - Jiyong Liang
View Article