验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells, mouse liver, rat liver |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human prostate cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy COG3 Ready-To-Use IHC Kit. COG3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IP | See 2 publications below |
产品信息
11130-1-AP targets COG3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | COG3 fusion protein Ag1610 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | component of oligomeric golgi complex 3 |
| 别名 | COG complex subunit 3, COG3, p94, SEC34 |
| 计算分子量 | 94 kDa |
| 观测分子量 | 94 kDa |
| GenBank蛋白编号 | BC038953 |
| 基因名称 | COG3 |
| Gene ID (NCBI) | 83548 |
| RRID | AB_2081402 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96JB2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COG3 antibody 11130-1-AP | Download protocol |
| IHC protocol for COG3 antibody 11130-1-AP | Download protocol |
| IP protocol for COG3 antibody 11130-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Cell The Genomic Landscape and Clinical Relevance of A-to-I RNA Editing in Human Cancers. | ||
Cancer Res Sustained Aurora Kinase B Expression Confers Resistance to PI3K Inhibition in Head and Neck Squamous Cell Carcinoma | ||
Sci Rep Characterization of the AGR2-NPM3 axis uncovers the AGR2 involvement in PD-L1 regulation in colorectal cancer |
