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验证数据展示

  • WB analysis using 10767-1-AP

    WB analysis using 10767-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10767-1-AP (COG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 10767-1-AP

    IHC staining of human ovary tumor using 10767-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10767-1-AP (COG1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COG1 antibody included
  • IHC staining of human ovary tumor using 10767-1-AP

    IHC staining of human ovary tumor using 10767-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10767-1-AP (COG1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with COG1 antibody included
  • WB analysis using 10767-1-AP

    WB analysis using 10767-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10767-1-AP (COG1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

COG1 Polyclonal antibody

COG1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10767-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CDG2G, COG complex subunit 1, COG1, KIAA1381, LDLB

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inU-251 cells, PC-3 cells, U2OS cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy COG1 Ready-To-Use IHC Kit. COG1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10767-1-AP targets COG1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1203

Product name: Recombinant human COG1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 561-980 aa of BC021985

Sequence: ADAGTVQEMLRTQSVACIKHIVDCIRAELQSIEEGVQGQQDALNSAKLHSVLFMARLCQSLGELCPHLKQCILGKSESSEKPAREFRALRKQGKVKTQEIIPTQAKWQEVKEVLLQQSVMGYQVWSSAVVKVLIHGFTQSLLLDDAGSVLATATSWDELEIQEEAESGSSVTSKIRLPAQPSWYVQSFLFSLCQEINRVGGHALPKVTLQEMLKSCMVQVVAAYEKLSEEKQIKKEGAFPVTQNRALQLLYDLRYLNIVLTAKGDEVKSGRSKPDSRIEKVTDHLEALIDPFDLDVFTPHLNSNLHRLVQRTSVLFGLVTGTENQLAPRSSTFNSQEPHNILPLASSQIRFGLLPLSMTSTRKAKSTRNIETKAQVVPPARSTAGDPTVPGSLFRQLVSEEDNTSAPSLFKLGWLSSMTK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 component of oligomeric golgi complex 1
别名 CDG2G, COG complex subunit 1, COG1, KIAA1381, LDLB
计算分子量 109 kDa
观测分子量 109 kDa
GenBank蛋白编号BC021985
基因名称 COG1
Gene ID (NCBI) 9382
RRIDAB_2083677
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WTW3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COG1 is one protein of the COG complex which is a heterooctameric complex that regulates intraGolgi trafficking and the integrity of the Golgi compartment in eukaryotic cells (PMID: 16537452). It is reported that this protein is required for steps in the normal medial and trans-Golgi-associated processing of glycoconjugates and plays a role in the organization of the Golgi-localized complex. COG1 has a calculated molecular weight of 109 kDa.

实验方案

Product Specific Protocols
IHC protocol for COG1 antibody 10767-1-APDownload protocol
WB protocol for COG1 antibody 10767-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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