验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
12991-1-AP targets COASY in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human, mouse, rat |
文献引用反应性 | rat |
免疫原 | COASY fusion protein Ag3651 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | Coenzyme A synthase |
别名 | DPCK, Dephosphocoenzyme A kinase, Dephospho-CoA pyrophosphorylase, Dephospho-CoA kinase, Coenzyme A synthase |
计算分子量 | 564 aa, 62 kDa |
观测分子量 | 62 kDa |
GenBank蛋白编号 | BC020985 |
基因名称 | COASY |
Gene ID (NCBI) | 80347 |
RRID | AB_2083528 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q13057 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Bifunctional coenzyme A synthase (COASY) is a bifunctional enzyme that catalyzes the fourth and fifth sequential steps of the CoA biosynthetic pathway. The fourth reaction is catalyzed by the phosphopantetheine adenylyltransferase, coded by the coaD domain; the fifth reaction is catalyzed by the dephospho-CoA kinase, coded by the coaE domain. May act as a point of CoA biosynthesis regulation. It was reported that having a key role in erythroid maturation and identified upstream and downstream metabolites of COASY as a potential treatment for anemia in patients with MDS-RS (PMID: 36857430). COASY has 2 isoforms with molecular weights of 62 and 65 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for COASY antibody 12991-1-AP | Download protocol |
IHC protocol for COASY antibody 12991-1-AP | Download protocol |
IF protocol for COASY antibody 12991-1-AP | Download protocol |
IP protocol for COASY antibody 12991-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |