验证数据展示
经过测试的应用
| Positive WB detected in | hTERT-RPE1 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34046-1-AP targets COA5 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag41138 Product name: Recombinant human COA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-74 aa of BC047722 Sequence: MPKYYEDKPQGGACAGLKEDLGACLLQSDCVVQEGKSPRQCLKEGYCNSLKYAFFECKRSVLDNRARFRGRKGY 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Cytochrome c oxidase assembly factor 5 |
| 别名 | C2orf64 |
| 观测分子量 | 8~12 kDa |
| GenBank蛋白编号 | BC047722 |
| 基因名称 | C2orf64 |
| Gene ID (NCBI) | 493753 |
| RRID | AB_3743142 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q86WW8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The cytochrome c oxidase assembly factor 5 (COA5) gene, previously denoted as C2orf64, was first reported in humans when a homozygous missense variant (c.157G>C, p.Ala53Pro) in this gene was shown to cause mitochondrial disease. COA5 is a small mitochondrial protein required for an early step in the assembly of cytochrome-c oxidase (complex IV) of the respiratory chain. It contains a conserved twin-CX9C motif, is located in the mitochondrial inner membrane (PMID: 39779219).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for COA5 antibody 34046-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
