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验证数据展示

  • WB analysis using 30721-1-AP

    WB analysis using 30721-1-AP

    LNCaP cells were subjected to SDS PAGE followed by western blot with 30721-1-AP (COA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 30721-1-AP

    IHC staining of human ovary cancer using 30721-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30721-1-AP (COA4 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COA4 Polyclonal antibody

COA4 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30721-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

CHCHD8, E2IG2, E2 induced gene 2 protein

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HEK-293 cells, HeLa cells, PC-3 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30721-1-AP targets COA4 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33966

Product name: Recombinant human CHCHD8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-87 aa of NM_016565.2

Sequence: MSTSVPQGHTWTQRVKKDDEEEDPLDQLISRSGCAASHFAVQECMAQHQDWRQCQPQVQAFKDCMSEQQARRQEELQRRQEQAGAHH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cytochrome c oxidase assembly factor 4 homolog, mitochondrial
别名 CHCHD8, E2IG2, E2 induced gene 2 protein
计算分子量10 kDa
观测分子量12 kDa
GenBank蛋白编号NM_016565.2
基因名称 COA4
Gene ID (NCBI) 51287
RRIDAB_3669754
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NYJ1-1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The coiled-coil-helix-coiled-coil-helix domain containing 8 (CHCHD8) is a putative COX assembly factor known as cytochrome c oxidase assembly factor 4 homolog (CoA4). COA4 is a newly identified CcO assembly factor which is a twin CX(9)C motif mitochondrial protein localized in the intermembrane space linked with the inner membrane of mitochondria. Its transport into intermembrane space depends on the MIA40 trans-site receptor machinery. (PMID: 37345060)

实验方案

Product Specific Protocols
IHC protocol for COA4 antibody 30721-1-APDownload protocol
WB protocol for COA4 antibody 30721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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