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验证数据展示

  • IF Staining of HeLa using 25197-1-AP

    IF Staining of HeLa using 25197-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using COA1 antibody (25197-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

COA1 Polyclonal antibody

COA1 Polyclonal Antibody for IF/ICC, ELISA
Cat No. 25197-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

C7orf44, MITRAC15, Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 15 kDa, Cytochrome c oxidase assembly factor 1 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25197-1-AP targets COA1 in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 COA1 fusion protein Ag18313 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Cytochrome c oxidase assembly factor 1 homolog
别名 C7orf44, MITRAC15, Mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein of 15 kDa, Cytochrome c oxidase assembly factor 1 homolog
计算分子量 146 aa, 17 kDa
GenBank蛋白编号BC056884
基因名称 COA1
Gene ID (NCBI) 55744
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9GZY4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COA1, also named as C7orf44 and MITRAC15, is a mitochondrial translation regulation assembly intermediate of cytochrome c oxidase protein with MW 15 kDa. It's a component of some MITRAC complex, a cytochrome c oxidase (COX) assembly intermediate complex that regulates COX assembly. COA1 is required for assembly of mitochondrial respiratory chain complex I and complex IV.

实验方案

Product Specific Protocols
IF protocol for COA1 antibody 25197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols