• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67061-1-Ig

    WB analysis using 67061-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67061-1-Ig (CNTROB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 67061-1-Ig

    IF Staining of hTERT-RPE1 using 67061-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CNTROB antibody (67061-1-Ig, Clone: 3D12A4 ) at dilution of 1:350 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).

CNTROB Monoclonal antibody

CNTROB Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67061-1-Ig

产品说明书

CloneNo. 3D12A4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, ELISA

别名

3D12A4, Centrobin, Centrosomal BRCA2-interacting protein, LIP8, LYST interacting protein 8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, pig brain tissue, rat brain tissue, NCI-H1299 cells, JAR cells, Jurkat cells, K-562 cells
Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy CNTROB Ready-To-Use IHC Kit. CNTROB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:175-1:700
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67061-1-Ig targets CNTROB in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 CNTROB fusion protein Ag25447 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 centrobin, centrosomal BRCA2 interacting protein
别名 3D12A4, Centrobin, Centrosomal BRCA2-interacting protein, LIP8, LYST interacting protein 8
计算分子量 903 aa, 101 kDa
观测分子量 97 kDa
GenBank蛋白编号BC021134
基因名称 CNTROB
Gene ID (NCBI) 116840
RRIDAB_2882371
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8N137
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CNTROB antibody 67061-1-IgDownload protocol
IF protocol for CNTROB antibody 67061-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols