验证数据展示
经过测试的应用
| Positive WB detected in | fetal human brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32250-1-AP targets CNTNAP4 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag37716 Product name: Recombinant human CNTNAP4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1135-1241 aa of NM_033401.4 Sequence: FSAVKSLVLGRILEHSDVDQDTALAGAQGFTGCLSAVQLSHVAPLKAALHPSHPDPVTVTGHVTESSCMAQPGTDATSRERTHSFADHSGTIDDREPLANAIKSDSA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | contactin associated protein-like 4 |
| 别名 | CASPR4, Cell recognition molecule Caspr4, Contactin-associated protein-like 4, KIAA1763 |
| 计算分子量 | 145kDa，1308aa / 137 kDa，1235aa |
| 观测分子量 | 150 kDa, 137 kDa |
| GenBank蛋白编号 | NM_033401.4 |
| 基因名称 | CNTNAP4 |
| Gene ID (NCBI) | 85445 |
| RRID | AB_3742553 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9C0A0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Contactin-associated protein-like 4 (Cntnap4) is critical for GABAergic transmission in the brain. Impaired Cntnap4 function is implicated in neurological disorders, such as autism. Loss of Cntnap4 causes pro-inflammatory cognitive decline (PMID: 37933376).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNTNAP4 antibody 32250-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
