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验证数据展示

  • WB analysis using 31698-1-AP (same clone as 31698-1-PBS)

    WB analysis using 31698-1-AP (same clone as 31698-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 31698-1-AP (CNTLN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31698-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 31698-1-AP (same clone as 31698-1-PBS)

    IHC staining of human liver using 31698-1-AP (same clone as 31698-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 31698-1-AP (CNTLN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31698-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 31698-1-AP (same clone as 31698-1-PBS)

    IF Staining of HeLa using 31698-1-AP (same clone as 31698-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CNTLN antibody (31698-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31698-1-PBS in a different storage buffer formulation.

CNTLN Polyclonal antibody, PBS Only

CNTLN Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31698-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

C9orf101, C9orf39, Centlein, Centrosomal protein

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31698-1-PBS targets CNTLN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36354

Product name: Recombinant human CNTLN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 150-380 aa of

Sequence: REKQKSEAKDRKVLEILQVKDAKIQEFEQRESVLKQEINDLVKRKIAVDEENAFLRKEFSDLEKKFKDKSQEIKDTKECVQNKEEQNRLVIKNLEEENKKLSTRCTDLLNDLEKLRKQEAHLRKEKYSTDAKIKTFEDNLIEARKEVEVSQSKYNALSLQLSNKQTELIQKDMDITLVRKELQELQNLYKQNSTHTAQQAELIQQLQVLNMDTQKVLRNQEDVHTAESISY

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 centlein, centrosomal protein
别名 C9orf101, C9orf39, Centlein, Centrosomal protein
计算分子量162kDa
观测分子量162 kDa
基因名称 CNTLN
Gene ID (NCBI) 54875
RRIDAB_3670084
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NXG0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CNTLN is also named C9orf101 and C9orf39. CNTLN is required for centrosome cohesion and recruitment of CEP68 to centrosomes. CNTLN is localized at the centrosome. The calculated molecular weight of CNTLN is 162 kDa.

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