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验证数据展示

  • WB analysis using 86967-4-RR (same clone as 86967-4-PBS)

    WB analysis using 86967-4-RR (same clone as 86967-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86967-4-RR (CNP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86967-4-PBS in a different storage buffer formulation.

  • WB analysis using 86967-4-RR (same clone as 86967-4-PBS)

    WB analysis using 86967-4-RR (same clone as 86967-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86967-4-RR (CNP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86967-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 86967-4-RR (same clone as 86967-4-PBS)

    IHC staining of mouse brain using 86967-4-RR (same clone as 86967-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 86967-4-RR (CNP antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86967-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 86967-4-RR (same clone as 86967-4-PBS)

    IF Staining of mouse brain using 86967-4-RR (same clone as 86967-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CNPase antibody (86967-4-RR, Clone: 252105D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86967-4-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 86967-4-RR (same clone as 86967-4-PBS)

    IF Staining of mouse brain using 86967-4-RR (same clone as 86967-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using CNPase antibody (86967-4-RR, Clone: 252105D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86967-4-PBS in a different storage buffer formulation.

CNPase Recombinant monoclonal antibody, PBS Only

CNPase Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86967-4-PBS

产品说明书

CloneNo. 252105D1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, Indirect ELISA

别名

CNP, 2',3' cyclic nucleotide 3' phosphodiesterase, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, EC:3.1.4.37

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86967-4-PBS targets CNPase in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4227

Product name: Recombinant human CNP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-421 aa of BC028040

Sequence: MNRGFSRKSHTFLPKIFFRKMSSSGAKDKPELQFPFLQDEDTVATLLECKTLFILRGLPGSGKSTLARVIVDKYRDGTKMVSADAYKITPGARGAFSEEYKRLDEDLAAYCRRRDIRILVLDDTNHERERLEQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSKAFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTII

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 2',3'-cyclic nucleotide 3' phosphodiesterase
别名 CNP, 2',3' cyclic nucleotide 3' phosphodiesterase, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, EC:3.1.4.37
计算分子量 421 aa, 48 kDa
观测分子量 45-48 kDa
GenBank蛋白编号BC028040
基因名称 CNPase
Gene ID (NCBI) 1267
RRIDAB_3745239
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09543
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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