验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive FC (Intra) detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13427-1-AP targets CNPase in WB, IHC, IF-P, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF-P, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 |
CatNo: Ag4227 Product name: Recombinant human CNP protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-421 aa of BC028040 Sequence: MNRGFSRKSHTFLPKIFFRKMSSSGAKDKPELQFPFLQDEDTVATLLECKTLFILRGLPGSGKSTLARVIVDKYRDGTKMVSADAYKITPGARGAFSEEYKRLDEDLAAYCRRRDIRILVLDDTNHERERLEQLFEMADQYQYQVVLVEPKTAWRLDCAQLKEKNQWQLSADDLKKLKPGLEKDFLPLYFGWFLTKKSSETLRKAGQVFLEELGNHKAFKKELRQFVPGDEPREKMDLVTYFGKRPPGVLHCTTKFCDYGKAPGAEEYAQQDVLKKSYSKAFTLTISALFVTPKTTGARVELSEQQLQLWPSDVDKLSPTDNLPRGSRAHITLGCAADVEAVQTGLDLLEILRQEKGGSRGEEVGELSRGKLYSLGNGRWMLTLAKNMEVRAIFTGYYGKGKPVPTQGSRKGGALQSCTII 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | 2',3'-cyclic nucleotide 3' phosphodiesterase |
| 别名 | CNP, 2',3' cyclic nucleotide 3' phosphodiesterase, 2',3'-cyclic-nucleotide 3'-phosphodiesterase, EC:3.1.4.37 |
| 计算分子量 | 421 aa, 48 kDa |
| 观测分子量 | 45-48 kDa |
| GenBank蛋白编号 | BC028040 |
| 基因名称 | CNPase |
| Gene ID (NCBI) | 1267 |
| RRID | AB_2082477 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P09543 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CNPase antibody 13427-1-AP | Download protocol |
| IF protocol for CNPase antibody 13427-1-AP | Download protocol |
| IHC protocol for CNPase antibody 13427-1-AP | Download protocol |
| WB protocol for CNPase antibody 13427-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Neurosci TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration. | ||
Nat Commun Phenylalanine-tRNA aminoacylation is compromised by ALS/FTD-associated C9orf72 C4G2 repeat RNA | ||
J Clin Invest Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling. | ||
Biomaterials Novel "hot spring"-mimetic scaffolds for sequential neurovascular network reconstruction and osteoporosis reversion | ||
Neural Regen Res Sodium selenite promotes neurological function recovery after spinal cord injury by inhibiting ferroptosis. | ||
Neural Regen Res Mechanisms underlying the promotion of functional recovery by deferoxamine after spinal cord injury in rats |
