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CNOT4 Polyclonal antibody

CNOT4 Polyclonal Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 12564-1-AP

Print datasheet

Synonyms

CCR4 associated factor 4, CLONE243, CNOT4, NOT4, NOT4H

验证数据展示

  • WB analysis of mouse skeletal muscle using 12564-1-AP

    WB analysis of mouse skeletal muscle using 12564-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12564-1-AP (CNOT4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of MCF-7 using 12564-1-AP

    WB analysis of MCF-7 using 12564-1-AP

    WB result of CNOT4 antibody (12564-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNOT4 transfected MCF-7 cells.

  • WB analysis using 12564-1-AP

    WB analysis using 12564-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12564-1-AP (CNOT4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 12564-1-AP

    IHC staining of human lung cancer using 12564-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 12564-1-AP (CNOT4 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with CNOT4 antibody included
  • IF Staining of HepG2 using 12564-1-AP

    IF Staining of HepG2 using 12564-1-AP

    Immunofluorescent analysis of HepG2 cells, using CNOT4 antibody 12564-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • FC experiment of HepG2 using 12564-1-AP

    FC experiment of HepG2 using 12564-1-AP

    1X10^6 HepG2 cells were stained with 0.2ug CNOT4 antibody (12564-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

经过测试的应用

Positive WB detected inmouse skeletal muscle tissue, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy CNOT4 Ready-To-Use IHC Kit. CNOT4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12564-1-AP targets CNOT4 in WB, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CNOT4 fusion protein Ag3163 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CCR4-NOT transcription complex, subunit 4
Synonyms CCR4 associated factor 4, CLONE243, CNOT4, NOT4, NOT4H
Calculated Molecular Weight 575 aa, 64 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC035590
Gene Symbol CNOT4
Gene ID (NCBI) 4850
RRIDAB_2082457
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95628
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CNOT4 antibody 12564-1-APDownload protocol
IHC protocol for CNOT4 antibody 12564-1-APDownload protocol
IF protocol for CNOT4 antibody 12564-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Cell Sci

Coupled protein quality control during nonsense-mediated mRNA decay

Authors - Alison J Inglis
View Article
humanWB,IF

Cells

Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability

Authors - Nafiseh Chalabi Hagkarim
  • KD Validated
View Article