验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, HEK-293T cells, HeLa cells |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CNOT4 Ready-To-Use IHC Kit. CNOT4 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12564-1-AP targets CNOT4 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | CNOT4 fusion protein Ag3163 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CCR4-NOT transcription complex, subunit 4 |
| 别名 | CCR4 associated factor 4, CCR4-associated factor 4, CCR4-NOT transcription complex subunit 4, CLONE243, E3 ubiquitin-protein ligase CNOT4 |
| 计算分子量 | 575 aa, 64 kDa |
| 观测分子量 | 64-85 kDa |
| GenBank蛋白编号 | BC035590 |
| 基因名称 | CNOT4 |
| Gene ID (NCBI) | 4850 |
| RRID | AB_2082457 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95628 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNOT4 antibody 12564-1-AP | Download protocol |
| IHC protocol for CNOT4 antibody 12564-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis | ||
Science Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex to translating ribosomes | ||
Cells Disruption of the Mammalian Ccr4-Not Complex Contributes to Transcription-Mediated Genome Instability
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