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CNN3 Polyclonal antibody

CNN3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 11509-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

CNN 3, Calponin-3, Calponin3, Calponin 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHCT 116 cells, HAP1 cells, human kidney tissue, human skeletal muscle tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells, U-87MG cells
Positive IHC detected inhuman prostate cancer tissue, human hysteromyoma tissue, human skin tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CNN3 Ready-To-Use IHC Kit. CNN3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11509-1-AP targets CNN3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 CNN3 fusion protein Ag2068 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calponin 3, acidic
别名 CNN 3, Calponin-3, Calponin3, Calponin 3
计算分子量 329 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC025372
基因名称 CNN3
Gene ID (NCBI) 1266
RRIDAB_2082140
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15417
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CNN3 antibody 11509-1-APDownload protocol
IHC protocol for CNN3 antibody 11509-1-APDownload protocol
IF protocol for CNN3 antibody 11509-1-APDownload protocol
IP protocol for CNN3 antibody 11509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Mol Ther Nucleic Acids

circRNA CDR1as Promotes Pulmonary Artery Smooth Muscle Cell Calcification by Upregulating CAMK2D and CNN3 via Sponging miR-7-5p.

Authors - Cui Ma
mouseIF

Cell Rep

Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.

Authors - Taha A Jan
humanWB

J Proteome Res

Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma

Authors - Pei-Shan Wu
mouseIHC

Mol Cell Biochem

STAT3 mediates ECM stiffness-dependent progression in ovarian cancer

Authors - Sun Chenchen
humanWB

Gastroenterol Res Pract

PMID: 30911294

Authors - Keun-Seok Hong
  • KD Validated
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