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  • Featured Product
  • KD/KO Validated

CNN3 Polyclonal antibody

CNN3 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 11509-1-AP

Print datasheet

Synonyms

Calponin 3, calponin 3, acidic, Calponin, acidic isoform, CNN3

验证数据展示

  • WB analysis of HEK-293 using 11509-1-AP

    WB analysis of HEK-293 using 11509-1-AP

    WB result of CNN3 antibody (11509-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN3 transfected HEK-293 cells.

  • WB analysis using 11509-1-AP

    WB analysis using 11509-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of human kidney using 11509-1-AP

    WB analysis of human kidney using 11509-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 11509-1-AP

    IF Staining of HeLa using 11509-1-AP

    Immunofluorescent analysis of Hela cells, using CNN3 antibody 11509-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • IHC staining of human prostate cancer using 11509-1-AP

    IHC staining of human prostate cancer using 11509-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CNN3 antibody included
  • IHC staining of human prostate cancer using 11509-1-AP

    IHC staining of human prostate cancer using 11509-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CNN3 antibody included
  • IP experiment of HEK-293 using 11509-1-AP

    IP experiment of HEK-293 using 11509-1-AP

    IP result of anti-CNN3 (IP:11509-1-AP, 4ug; Detection:11509-1-AP 1:15000) with HEK-293 cells lysate 960 ug.

经过测试的应用

Positive WB detected inHCT 116 cells, HAP1 cells, human kidney tissue, human skeletal muscle tissue, HEK-293 cells, HepG2 cells, NIH/3T3 cells
Positive IP detected inHEK-293 cells, U-87MG cells
Positive IHC detected inhuman prostate cancer tissue, human skin tissue, human hysteromyoma tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy CNN3 Ready-To-Use IHC Kit. CNN3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11509-1-AP targets CNN3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen CNN3 fusion protein Ag2068 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name calponin 3, acidic
Synonyms Calponin 3, calponin 3, acidic, Calponin, acidic isoform, CNN3
Calculated Molecular Weight 329 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC025372
Gene Symbol CNN3
Gene ID (NCBI) 1266
RRIDAB_2082140
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ15417
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CNN3 antibody 11509-1-APDownload protocol
IHC protocol for CNN3 antibody 11509-1-APDownload protocol
IF protocol for CNN3 antibody 11509-1-APDownload protocol
IP protocol for CNN3 antibody 11509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Cell Rep

Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.

Authors - Taha A Jan
View Article
humanWB

J Proteome Res

Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma

Authors - Pei-Shan Wu
View Article
humanWB

Gastroenterol Res Pract

PMID: 30911294

Authors - Keun-Seok Hong
  • KD Validated
View Article