CNKSR3 Polyclonal antibody
CNKSR3 Polyclonal Antibody for IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
14084-1-AP targets CNKSR3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IP, WB,ELISA |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | CNKSR3 fusion protein Ag5213 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | CNKSR family member 3 |
| Synonyms | CNK homolog protein 3, CNK3, CNKSR family member 3, CNKSR3, Connector enhancer of KSR 3, MAGI1, Maguin like protein |
| Calculated Molecular Weight | 555 aa, 62 kDa |
| Observed Molecular Weight | 62-65 kDa |
| GenBank Accession Number | BC060761 |
| Gene Symbol | CNKSR3 |
| Gene ID (NCBI) | 154043 |
| RRID | AB_2229651 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6P9H4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Connector enhancer of kinase suppressor of ras 3 (CNKSR3) gene encodes CNK homolog protein 3 (CNK3). CNK proteins, originally identified in Drosophila, were described to regulate the RAS/ERK pathway at the level of the RAS protein kinase. CNKSR3, homologue of scaffold proteins involved in MAPK pathway regulation, is a direct target of mineralocorticoid receptor (MR). CNK3 coordinates the assembly of a multiprotein epithelial sodium channel (ENaC) -regulatory complex, and hence plays a central role in sodium homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CNKSR3 antibody 14084-1-AP | Download protocol |
| IP protocol for CNKSR3 antibody 14084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Biol Chem Scaffold protein connector enhancer of kinase suppressor of Ras isoform 3 (CNK3) coordinates assembly of a multiprotein epithelial sodium channel (ENaC)-regulatory complex. |
