验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, mouse brain tissue |
| Positive IP detected in | mouse brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
14084-1-AP targets CNKSR3 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag5213 Product name: Recombinant human CNKSR3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-352 aa of BC060761 Sequence: MEPVTKWSPKQVVDWTRGLDDCLQQYVHKFEREKINGEQLLQISHQDLEELGVTRIGHQELVLEAVDLLCALNYGLETDNMKNLVLKLRASSHNLQNYISSRRKSPAYDGNTSRKAPNEFLTSVVELIGAAKALLAWLDRAPFTGITDFSVTKNKIIQLCLDLTTTVQKDCFVAEMEDKVLTVVKVLNGICDKTIRSTTDPVMSQCACLEEVHLPNIKPGEGLGMYIKSTYDGLHVITGTTENSPADRSQKIHAGDEVIQVNQQTVVGWQLKNLVKKLRENPTGVVLLLKKRPTGSFNFTPAPLKNLRWKPPLVQTSPPPATTQSPESTMDTSLKKEKSAILDLYIPPPPAV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CNKSR family member 3 |
| 别名 | CNK homolog protein 3, CNK3, CNKSR family member 3, CNKSR3, Connector enhancer of KSR 3, MAGI1, Maguin like protein |
| 计算分子量 | 555 aa, 62 kDa |
| 观测分子量 | 62-65 kDa |
| GenBank蛋白编号 | BC060761 |
| 基因名称 | CNKSR3 |
| Gene ID (NCBI) | 154043 |
| RRID | AB_2229651 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q6P9H4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Connector enhancer of kinase suppressor of ras 3 (CNKSR3) gene encodes CNK homolog protein 3 (CNK3). CNK proteins, originally identified in Drosophila, were described to regulate the RAS/ERK pathway at the level of the RAS protein kinase. CNKSR3, homologue of scaffold proteins involved in MAPK pathway regulation, is a direct target of mineralocorticoid receptor (MR). CNK3 coordinates the assembly of a multiprotein epithelial sodium channel (ENaC) -regulatory complex, and hence plays a central role in sodium homeostasis.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for CNKSR3 antibody 14084-1-AP | Download protocol |
| WB protocol for CNKSR3 antibody 14084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
