• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CNKSR1 Polyclonal antibody

CNKSR1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 10885-1-AP

Print datasheet

Synonyms

CNK, CNK homolog protein 1, CNK1, CNKSR1, Connector enhancer of KSR 1, Connector enhancer of KSR like, hCNK1, KSR

验证数据展示

  • WB analysis of HT-29 using 10885-1-AP

    WB analysis of HT-29 using 10885-1-AP

    HT-29 cells were subjected to SDS PAGE followed by western blot with 10885-1-AP (CNKSR1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 10885-1-AP

    IHC staining of human pancreas cancer using 10885-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10885-1-AP (CNKSR1 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 10885-1-AP

    IHC staining of human pancreas cancer using 10885-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10885-1-AP (CNKSR1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HT-29 using 10885-1-AP

    IP experiment of HT-29 using 10885-1-AP

    IP result of anti-CNKSR1 (IP:10885-1-AP, 4ug; Detection:10885-1-AP 1:1000) with HT-29 cells lysate 4000 ug.

经过测试的应用

Positive WB detected inHT-29 cells, human brain tissue
Positive IP detected inHT-29 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10885-1-AP targets CNKSR1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen CNKSR1 fusion protein Ag1333 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name connector enhancer of kinase suppressor of Ras 1
Synonyms CNK, CNK homolog protein 1, CNK1, CNKSR1, Connector enhancer of KSR 1, Connector enhancer of KSR like, hCNK1, KSR
Calculated Molecular Weight 80 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC011604
Gene Symbol CNKSR1
Gene ID (NCBI) 10256
RRIDAB_2276387
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ969H4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNKSR1 is a protein containing several motifs involved in protein-protein interaction, including PDZ, PH (Pleckstrin homology), and SAM (sterile alpha motif) domains. The encoded protein acts as a scaffold component for receptor tyrosine kinase signaling and may mediate crosstalk between different signaling pathways. CNKSR1 is required in multiple receptor tyrosine kinase pathways where it appears to be a tyrosine phosphorylation targe.

实验方案

Product Specific Protocols
WB protocol for CNKSR1 antibody 10885-1-APDownload protocol
IHC protocol for CNKSR1 antibody 10885-1-APDownload protocol
IP protocol for CNKSR1 antibody 10885-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Life Sci Alliance

CNKSR1 serves as a scaffold to activate an EGFR phosphatase via exclusive interaction with RhoB-GTP.

Authors - Kanako Nishiyama
  • KD Validated
View Article
humanIHC

Cancer Rep (Hoboken)

Identification of hub genes in bladder cancer based on weighted gene co-expression network analysis from TCGA database.

Authors - Lei Wang
View Article