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验证数据展示

  • WB analysis using 15015-1-AP

    WB analysis using 15015-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15015-1-AP (CNIH4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of mouse testis using 15015-1-AP

    IF Staining of mouse testis using 15015-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CNIH4 antibody (15015-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse testis using 15015-1-AP

    IF Staining of mouse testis using 15015-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CNIH4 antibody (15015-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CNIH4 Polyclonal antibody

CNIH4 Polyclonal Antibody for WB, IF-P, ELISA
Cat No. 15015-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, ELISA and More (1)

别名

CNIH-4, Cornichon family AMPA receptor auxiliary protein 4, HSPC163, Protein cornichon homolog 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IF-P detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15015-1-AP targets CNIH4 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 CNIH4 fusion protein Ag6965 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cornichon homolog 4 (Drosophila)
别名 CNIH-4, Cornichon family AMPA receptor auxiliary protein 4, HSPC163, Protein cornichon homolog 4
计算分子量 16 kDa
观测分子量16-18 kDa
GenBank蛋白编号BC000573
基因名称 CNIH4
Gene ID (NCBI) 29097
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P003
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNIH4 (Cornichon Family Member 4) is a protein-coding gene belonging to the cornichon family, which plays a critical role in regulating G protein-coupled receptor (GPCR) trafficking from the endoplasmic reticulum to the cell surface. It facilitates GPCR export through the early secretory pathway and is regulated by TMED9.

实验方案

Product Specific Protocols
WB protocol for CNIH4 antibody 15015-1-APDownload protocol
IF protocol for CNIH4 antibody 15015-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

World J Gastroenterol

Genome-wide map of N6-methyladenosine circular RNAs identified in mice model of severe acute pancreatitis.

Authors - Jun Wu
View Article
humanIHC

Biomedicines

CLIC4 Is a New Biomarker for Glioma Prognosis

Authors - Zhichun Liu
View Article