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验证数据展示

  • IHC staining of mouse brain using 14260-1-AP

    IHC staining of mouse brain using 14260-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14260-1-AP (CNIH2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 14260-1-AP

    IF Staining of A549 using 14260-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CNIH2 antibody (14260-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

CNIH2 Polyclonal antibody

CNIH2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 14260-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

Protein cornichon homolog 2, Cornichon-like protein, Cornichon like protein, Cornichon family AMPA receptor auxiliary protein 2, CNIL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14260-1-AP targets CNIH2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CNIH2 fusion protein Ag5533 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cornichon homolog 2 (Drosophila)
别名 Protein cornichon homolog 2, Cornichon-like protein, Cornichon like protein, Cornichon family AMPA receptor auxiliary protein 2, CNIL
计算分子量 19 kDa
GenBank蛋白编号BC047953
基因名称 CNIH2
Gene ID (NCBI) 254263
RRIDAB_3669181
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PI25
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CNIH2, or cornichon family AMPA receptor auxiliary protein 2, is an auxiliary subunit of the ionotropic glutamate receptor of the AMPA subtype.CNIH2 has been reported to interact with the Type I AMPA Receptor regulatory protein isoform gamma-8, which controls the assembly of hippocampal AMPA receptor complexes, thereby modulating receptor gating and pharmacology. It has been implicated in various diseases, including schizophrenia, as indicated by its association with different schizophrenia-related disorders.

实验方案

Product Specific Protocols
IHC protocol for CNIH2 antibody 14260-1-APDownload protocol
IF protocol for CNIH2 antibody 14260-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols