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验证数据展示

  • WB analysis using 86475-1-RR (same clone as 86475-1-PBS)

    WB analysis using 86475-1-RR (same clone as 86475-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86475-1-RR (CMPK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86475-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA sample test of MP02460-1

    Sandwich ELISA sample test of MP02460-1

    The mean UMP/CMP kinase concentration was determined to be 383.79 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load and 348.74 ng/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load.

  • Sandwich ELISA standard curve of MP02460-1

    Sandwich ELISA standard curve of MP02460-1

    Sandwich ELISA standard curve of MP02460-1, Human UMP/CMP kinase Recombinant Matched Antibody Pair - PBS only. 86475-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1920. 86475-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

  • IHC staining of human lung cancer using 86475-1-RR (same clone as 86475-1-PBS)

    IHC staining of human lung cancer using 86475-1-RR (same clone as 86475-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 86475-1-RR (CMPK1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86475-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CMPK1 antibody included
  • IF Staining of HeLa using 86475-1-RR (same clone as 86475-1-PBS)

    IF Staining of HeLa using 86475-1-RR (same clone as 86475-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CMPK1 antibody (86475-1-RR, Clone: 251233D3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86475-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02460-1

    Cytometric bead array sample test of MP02460-1

    The mean UMP/CMP kinase concentration was determined to be 134.2 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02460-1

    Cytometric bead array standard curve of MP02460-1

    Cytometric bead array standard curve of MP02460-1, UMP/CMP kinase Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86475-2-PBS. Detection antibody: 86475-1-PBS. Standard: Ag1920. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 86475-1-RR

    Affinity and Kinetic Characterization of 86475-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86475-1-RR against Human UMP/CMP kinase were performed. The affinity constant is below 1 pM.

UMP/CMP kinase Recombinant monoclonal antibody, PBS Only (Detector)

UMP/CMP kinase Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA
Cat No. 86475-1-PBS

产品说明书

CloneNo. 251233D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA

别名

CMPK1, CK, CMK, CMPK, Cytidine monophosphate kinase

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CMPK1 Ready-To-Use IHC Kit. CMPK1 primary antibody included.

产品信息

86475-1-PBS targets UMP/CMP kinase as part of a matched antibody pair:

MP02460-1: 86475-2-PBS capture and 86475-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1920

Product name: Recombinant human CMPK1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-228 aa of BC014961

Sequence: MLSRCRSRLLHVLGLSFLLQTRRPILLCSPRLMKPLVVFVLGGPGAGKGTQCARIVEKYGYTHLSAGELLRDERKNPDSQYGELIEKYIKEGKIVPVEITISLLKREMDQTMAANAQKNKFLIDGFPRNQDNLQGWNKTMDGKADVSFVLFFDCNNEICIERCLERGKSSGRSDDNRESLEKRIQTYLQSTKPIIDLYEEMGKVKKIDASKSVDEVFDEVVQIFDKEG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
别名 CMPK1, CK, CMK, CMPK, Cytidine monophosphate kinase
计算分子量 228 aa, 26 kDa
观测分子量 22 kDa
GenBank蛋白编号BC014961
基因名称 CMPK1
Gene ID (NCBI) 51727
RRIDAB_3744911
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP30085
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Pyrimidine nucleoside monophosphate kinase [UMP/CMP kinase (UMP/CMPK)] (CMPK1) is also named as CMK, CMPK, UCK, UMK, UMPK and belongs to the adenylate kinase family. It plays a crucial role in the formation of UDP, CDP, and dCDP, which are required for cellular nucleic acid synthesis(PMID:11912132). CMPK1 can use a broad spectrum of phosphate donors but the best phosphate donors are ATP and dATP. CMPK1 has been reported as a prognostic marker for non-small cell lung cancer, pancreatic cancer and breast cancer (PMID: 21642870, 22838950, 27558661). The longest deduced protein contains 228 amino acids and has a calculated molecular mass of 26 kD. However, translation likely begins at the second initiation methionine, resulting in a protein of 196 amino acids(PMID:10462544). The shorter form is the endogenously translated protein(PMID:11912132). CMPK1 has 3 isoforms with the molecular mass of 22, 16 and 26 kDa.

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