验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, human heart tissue, Jurkat cells, THP-1 cells |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy CLYBL Ready-To-Use IHC Kit. CLYBL primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
17314-1-AP targets CLYBL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | mouse |
| 免疫原 | CLYBL fusion protein Ag11283 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | citrate lyase beta like |
| 别名 | citrate lyase beta like, CLB, CLYBL |
| 计算分子量 | 340 aa, 37 kDa |
| 观测分子量 | 37 kDa |
| GenBank蛋白编号 | BC034360 |
| 基因名称 | CLYBL |
| Gene ID (NCBI) | 171425 |
| RRID | AB_2083483 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8N0X4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CLYBL, also named as CLB, is a Mitochondrial citramalyl-CoA lyase indirectly involved in the vitamin B12 metabolism (PubMed:29056341). It is a human mitochondrial enzyme of unknown function that is found in multiple eukaryotic taxa and conserved to bacteria. It acts as a beta-methylmalate synthase in vitro, by mediating conversion of glyoxylate and propionyl-CoA to beta-methylmalate. There're two isoforms with MW 31-33 kDa and 35-37 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CLYBL antibody 17314-1-AP | Download protocol |
| IHC protocol for CLYBL antibody 17314-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Death Dis Positive feedback loop involving AMPK and CLYBL acetylation links metabolic rewiring and inflammatory responses | ||
Eur J Immunol YY1 control of mitochondrial-related genes does not account for regulation of immunoglobulin class switch recombination in mice. |
