验证数据展示
经过测试的应用
| Positive WB detected in | ARPE-19 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27498-1-AP targets CLUL1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag26570 Product name: Recombinant human CLUL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 21-124 aa of BC025381 Sequence: APTWKDKTAISENLKSFSEVGEIDADEEVKKALTGIKQMKIMMERKEKEHTNLMSTLKKCREEKQEALKLLNEVQEHLEEEERLCRESLADSWGECRSCLENNC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | clusterin-like 1 (retinal) |
| 别名 | Retinal-specific clusterin-like protein, Clusterin-like protein 1, Clusterin like protein 1, clusterin like 1 (retinal) |
| 计算分子量 | 54 kDa |
| 观测分子量 | 70 kDa |
| GenBank蛋白编号 | BC025381 |
| 基因名称 | CLUL1 |
| Gene ID (NCBI) | 27098 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15846 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Clusterin-like protein 1 (CLUL1) functions as a molecular chaperone, protecting stressed proteins, and is upregulated in various neurodegenerative conditions, suggesting a role in defense against neuronal damage (PMID: 17148042). It is specifically expressed in cone photoreceptor cells and is regulated by the cone-rod homeobox (CRX) (PMID: 14507903). Mutations of CLUL1 were investigated in the context of age-related macular degeneration (AMD), a leading cause of blindness in the elderly (PMID: 17148042).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CLUL1 antibody 27498-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
