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验证数据展示

  • WB analysis using 86660-3-RR

    WB analysis using 86660-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86660-3-RR (CLTC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IP experiment of HEK-293 using 86660-3-RR

    IP experiment of HEK-293 using 86660-3-RR

    IP result of anti-CLTC (IP:86660-3-RR, 4ug; Detection:86660-3-RR 1:2000) with HEK-293 cells lysate 1200 ug.

  • IHC staining of human colon cancer using 86660-3-RR

    IHC staining of human colon cancer using 86660-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86660-3-RR (CLTC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLTC antibody included
  • IHC staining of human colon cancer using 86660-3-RR

    IHC staining of human colon cancer using 86660-3-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 86660-3-RR (CLTC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLTC antibody included
  • IF Staining of HEK-293 using 86660-3-RR

    IF Staining of HEK-293 using 86660-3-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using CLTC antibody (86660-3-RR, Clone: 251634B7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86660-3-RR

    Affinity and Kinetic Characterization of 86660-3-RR

    Biolayer interferometry (BLl) kinetic assays of 86660-3-RR against Human CLTC were performed. The affinity constant is below 1 pM.

CLTC Recombinant monoclonal antibody

CLTC Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 86660-3-RR

产品说明书

CloneNo. 251634B7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IP, ELISA

别名

CHC, CHC17, Clathrin heavy chain, Clathrin heavy chain 1, clathrin, heavy chain (Hc)

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, Raji cells, mouse brain tissue, rat brain tissue, pig brain tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy CLTC Ready-To-Use IHC Kit. CLTC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86660-3-RR targets CLTC in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag25041

Product name: Recombinant human CLTC protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC054489

Sequence: MAQILPIRFQEHLQLQNLGINPANIGFSTLTMESDKFICIREKVGEQAQVVIIDMNDPSNPIRRPISADSAIMNPASKVIALKAGKTLQIFNIEMKSKMKAHTMTDDVTFWKWISLNTVALVTDNAVYHWSMEGESQPVKMFDRHSSLAGCQIINYRTDAKQKWLLLTGISAQQNRVVGAMQLYSVDRKVSQPIEGHAASFAQFKMEGNAEESTLFCFAVRGQAGGKLHIIEVGTPPTGNQPFPKKAVDVFFPPEAQNDFPVAMQISEKHDVVFLITKYGYIHLYDLETGTCIYMNRISGETIFVTAPHEATAGIIGVNRKGQVLSVCVEEENIIPYITNVLQNPDLALRM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 clathrin, heavy chain (Hc)
别名 CHC, CHC17, Clathrin heavy chain, Clathrin heavy chain 1, clathrin, heavy chain (Hc)
计算分子量 192 kDa
观测分子量180 kDa
GenBank蛋白编号BC054489
基因名称 CLTC
Gene ID (NCBI) 1213
RRIDAB_3745036
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ00610
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)

实验方案

Product Specific Protocols
IF protocol for CLTC antibody 86660-3-RRDownload protocol
IHC protocol for CLTC antibody 86660-3-RRDownload protocol
IP protocol for CLTC antibody 86660-3-RRDownload protocol
WB protocol for CLTC antibody 86660-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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