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验证数据展示

  • WB analysis of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)

    WB analysis of HeLa using 66487-1-Ig (same clone as 66487-1-PBS)

    WB result of CLTC antibody (66487-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLTC transfected HeLa cells. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • WB analysis using 66487-1-Ig (same clone as 66487-1-PBS)

    WB analysis using 66487-1-Ig (same clone as 66487-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66487-1-Ig (CLTC antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLTC antibody included
  • IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    IHC staining of mouse kidney using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66487-1-Ig (CLTC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CLTC antibody included
  • IF Staining of HepG2 using 66487-1-Ig (same clone as 66487-1-PBS)

    IF Staining of HepG2 using 66487-1-Ig (same clone as 66487-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CLTC antibody (66487-1-Ig, Clone: 1B3D7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66487-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CLTC Monoclonal antibody, PBS Only

CLTC Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66487-1-PBS

产品说明书

CloneNo. 1B3D7

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig, rabbit

应用

WB, IHC, IF/ICC, ELISA

别名

1B3D7, CHC, CHC17, Clathrin heavy chain, Clathrin heavy chain 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CLTC Ready-To-Use IHC Kit. CLTC primary antibody included.

产品信息

66487-1-PBS targets CLTC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, pig, rabbit
免疫原 CLTC fusion protein Ag25040 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 clathrin, heavy chain (Hc)
别名 1B3D7, CHC, CHC17, Clathrin heavy chain, Clathrin heavy chain 1
计算分子量 192 kDa
观测分子量 170 kDa
GenBank蛋白编号BC054489
基因名称 CLTC
Gene ID (NCBI) 1213
RRIDAB_2881852
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ00610
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. The clathrin molecule has a triskelion shape. Each clathrin triskelion is composed of three identical heavy chains (CLTC) (160-190 kDa) and three light chains of two types, LCA (CLTA) and LCB (CLTB) (30-40 kDa). The heavy chain provides the structural backbone of the clathrin lattice. (PMID: 9133677)