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验证数据展示

  • WB analysis using 11766-1-AP

    WB analysis using 11766-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11766-1-AP (CLPS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat pancreas using 11766-1-AP

    IHC staining of rat pancreas using 11766-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 11766-1-AP (CLPS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat pancreas using 11766-1-AP

    IHC staining of rat pancreas using 11766-1-AP

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 11766-1-AP (CLPS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse pancreas using 11766-1-AP

    IF Staining of mouse pancreas using 11766-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse pancreas tissue using CLPS antibody (11766-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CLPS Polyclonal antibody

CLPS Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 11766-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

Colipase

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse pancreas tissue, rat pancreas tissue
Positive IHC detected inrat pancreas tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse pancreas tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11766-1-AP targets CLPS in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2321

Product name: Recombinant human CLPS protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-112 aa of BC017897

Sequence: MEKILILLLVALSVAYAAPGPRGIIINLENGELCMNSAQCKSNCCQHSSALGLARCTSMASENSECSVKTLYGIYYKCPCERGLTCEGDKTIVGSITNTNFGICHDAGRSKQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 colipase, pancreatic
别名 Colipase
计算分子量 12 kDa
观测分子量12 kDa
GenBank蛋白编号BC017897
基因名称 CLPS
Gene ID (NCBI) 1208
RRIDAB_10863117
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04118
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Pancreatic colipase is a 12-kDa polypeptide cofactor for pancreatic lipase (EC 3.1.1.3), an enzyme essential for the absorption of dietary long-chain triglyceride fatty acids. Colipase is thought to anchor lipase noncovalently to the surface of lipid micelles, counteracting the destabilizing influence of intestinal bile salts.

实验方案

Product Specific Protocols
IF protocol for CLPS antibody 11766-1-APDownload protocol
IHC protocol for CLPS antibody 11766-1-APDownload protocol
WB protocol for CLPS antibody 11766-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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