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验证数据展示

  • IHC staining of mouse liver using 30680-1-AP

    IHC staining of mouse liver using 30680-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30680-1-AP (CLOCK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLOCK antibody included
  • IHC staining of mouse liver using 30680-1-AP

    IHC staining of mouse liver using 30680-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30680-1-AP (CLOCK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CLOCK antibody included
  • IF Staining of HeLa using 30680-1-AP

    IF Staining of HeLa using 30680-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLOCK antibody (30680-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

CLOCK Polyclonal antibody

CLOCK Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 30680-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

IHC, IF/ICC, ELISA

别名

bHLHe8, CLOCK, clock homolog (mouse), hCLOCK, KAT13D, KIAA0334

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CLOCK Ready-To-Use IHC Kit. CLOCK primary antibody included.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30680-1-AP targets CLOCK in IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 CLOCK fusion protein Ag33370 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 clock homolog (mouse)
别名 bHLHe8, CLOCK, clock homolog (mouse), hCLOCK, KAT13D, KIAA0334
计算分子量 846 aa, 95 kDa
GenBank蛋白编号BC041878
基因名称 CLOCK
Gene ID (NCBI) 9575
RRIDAB_3669749
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15516
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Circadian locomoter output cycles protein kaput (CLOCK), also named as BHLHE8 or KIAA0334, is a 846 amino acid protein, which contains one bHLH domain, one PAC domain, and two PAS domains. CLOCK localizes in the nucleus and cytoplasm. CLOCK) is expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. ARNTL/2-CLOCK heterodimers activate E-box element (5'-CACGTG-3') transcription of a number of proteins of the circadian clock, such as PER1 and PER2. western blot. Our result is similar to the result of reseach paper (PMID: 30683868).

实验方案

Product Specific Protocols
IHC protocol for CLOCK antibody 30680-1-APDownload protocol
IF protocol for CLOCK antibody 30680-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols