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验证数据展示

  • WB analysis using 85378-1-RR

    WB analysis using 85378-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85378-1-RR (CLN5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 85378-1-RR

    IF Staining of A431 using 85378-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CLN5 antibody (85378-1-RR, Clone: 242812H6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

CLN5 Recombinant antibody

CLN5 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85378-1-RR

产品说明书

CloneNo. 242812H6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Ceroid lipofuscinosis neuronal protein 5, BMPS, BMP synthase CLN5, Bis(monoacylglycero)phosphate synthase CLN5, secreted form, Bis(monoacylglycero)phosphate synthase CLN5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HepG2 cells, A549 cells, HEK-293T cells, NIH/3T3 cells
Positive IF/ICC detected inA431 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85378-1-RR targets CLN5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 CLN5 fusion protein Ag18175 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 ceroid-lipofuscinosis, neuronal 5
别名 Ceroid lipofuscinosis neuronal protein 5, BMPS, BMP synthase CLN5, Bis(monoacylglycero)phosphate synthase CLN5, secreted form, Bis(monoacylglycero)phosphate synthase CLN5
计算分子量 407 aa, 46 kDa
观测分子量48 kDa
GenBank蛋白编号BC153154
基因名称 CLN5
Gene ID (NCBI) 1203
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75503
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The CLN5 (ceroid lipofuscinosis neuronal protein 5) protein is ubiquitously expressed in the majority of tissues studied and in the brain, CLN5 shows both neuronal and glial cell expression. CLN5 plays a role in maintaining an acidic environment in the lysosomes, a critical feature for a functional lysosome (PMID: 38236309). Moreover, CLN5-deficient neuronal progenitor cells showed reduced thioesterase activity, confirming the live cell function of CLN5 in setting S-depalmitoylation levels (PMID: 35427157).

实验方案

Product Specific Protocols
WB protocol for CLN5 antibody 85378-1-RRDownload protocol
IF protocol for CLN5 antibody 85378-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols