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验证数据展示

  • WB analysis using 67957-1-Ig

    WB analysis using 67957-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67957-1-Ig (CLN3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

CLN3 Monoclonal antibody

CLN3 Monoclonal Antibody for WB, ELISA
Cat No. 67957-1-Ig

产品说明书

CloneNo. 1E10A9

宿主/亚型

Mouse / IgG1

种属反应性

Human

应用

WB, ELISA

别名

Batten disease protein, BATTENIN, BTS, CLN3, JNCL, Protein CLN3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, NCCIT cells, NCI-H1299 cells, A549 cells, Jurkat cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67957-1-Ig targets CLN3 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag31402

Product name: Recombinant human CLN3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 300-438 aa of BC002394

Sequence: QGLFELLFFWNTSLSHAQQYRWYQMLYQAGVFASRSSLRCCRIRFTWALALLQCLNLVFLLADVWFGFLPSIYLVFLIILYEGLLGGAAYVNTFHNIALETSDEHREFAMAATCISDTLGISLSGLLALPLHDFLCQLS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ceroid-lipofuscinosis, neuronal 3
别名 Batten disease protein, BATTENIN, BTS, CLN3, JNCL, Protein CLN3
计算分子量 438 aa, 48 kDa
观测分子量50 kDa
GenBank蛋白编号BC002394
基因名称 CLN3
Gene ID (NCBI) 1201
RRIDAB_2918708
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ13286
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Neuronal ceroid lipofuscinosis (NCL, or Batten disease) refers to a group of lethal pediatric neurodegenerative diseases originating from mutations in one of the thus far identified 13 CLN genes (Ceroid Lipofuscinosis, Neuronal type; CLN1 to CLN14) (PMID: 25051496). CLN3 is a multi-membrane-spanning protein involved in the microtubule-dependent, anterograde transport of late endosomes and lysosomes. The CLN3 gene is located on chromosome 16p12.1 and produces three mRNA splicing variants. The 438-amino-acid CLN3 protein has a calculated molecular weight of 48 kDa. It has been reported that CLN3 can be glycosylated and form a homodimeric complex (PMID: 10356317; 17286803).

实验方案

Product Specific Protocols
WB protocol for CLN3 antibody 67957-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols