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验证数据展示

  • WB analysis of mouse cerebellum using 67776-1-Ig

    WB analysis of mouse cerebellum using 67776-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 67776-1-Ig

    WB analysis of A549 using 67776-1-Ig

    WB result of CLIP-115/CLIP2 antibody (67776-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIP-115/CLIP2 transfected A549 cells.

  • IF Staining of A549 using 67776-1-Ig

    IF Staining of A549 using 67776-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CLIP-115/CLIP2 antibody (67776-1-Ig, Clone: 2E6B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

CLIP-115/CLIP2 Monoclonal antibody

CLIP-115/CLIP2 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67776-1-Ig

产品说明书

CloneNo. 2E6B6

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat, pig, rabbit

应用

WB, IF/ICC, ELISA

别名

CLIP, CLIP 115, CLIP-115/CLIP2, CLIP2, CYLN2, Cytoplasmic linker protein 115, Cytoplasmic linker protein 2, KIAA0291, WBSCR3, WBSCR4, WSCR3, WSCR4

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse cerebellum tissue, rabbit brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, A549 cells
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy CLIP2 Ready-To-Use IHC Kit. CLIP2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67776-1-Ig targets CLIP-115/CLIP2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat, pig, rabbit
免疫原 CLIP-115/CLIP2 fusion protein Ag18902 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 CAP-GLY domain containing linker protein 2
别名 CLIP, CLIP 115, CLIP-115/CLIP2, CLIP2, CYLN2, Cytoplasmic linker protein 115, Cytoplasmic linker protein 2, KIAA0291, WBSCR3, WBSCR4, WSCR3, WSCR4
计算分子量 1011 aa, 112 kDa
观测分子量120 kDa
GenBank蛋白编号BC150332
基因名称 CLIP2
Gene ID (NCBI) 7461
RRIDAB_2918541
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9UDT6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CILP-2/CLIP-115 is a cytoplasmic linker protein (CLIP) that links membranous organelles to microtubules. CLIP-115 is enriched in the axonal growth cone and it enables neuronal polarization by controlling the stabilization of microtubules. Mutation of CLIP-115 has been associated with Williams-Beuren syndrome.

实验方案

Product Specific Protocols
WB protocol for CLIP-115/CLIP2 antibody 67776-1-IgDownload protocol
IF protocol for CLIP-115/CLIP2 antibody 67776-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols