• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

CLIP-115/CLIP2 Monoclonal antibody

CLIP-115/CLIP2 Monoclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig, rabbit

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E6B6

Cat No : 67776-1-Ig

Print datasheet

Synonyms

CLIP, CLIP 115, CLIP-115/CLIP2, CLIP2, CYLN2, Cytoplasmic linker protein 115, Cytoplasmic linker protein 2, KIAA0291, WBSCR3, WBSCR4, WSCR3, WSCR4

验证数据展示

  • WB analysis of mouse cerebellum using 67776-1-Ig

    WB analysis of mouse cerebellum using 67776-1-Ig

    mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 67776-1-Ig (CLIP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of A549 using 67776-1-Ig

    WB analysis of A549 using 67776-1-Ig

    WB result of CLIP-115/CLIP2 antibody (67776-1-Ig; 1:800; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIP-115/CLIP2 transfected A549 cells.

  • IF Staining of A549 using 67776-1-Ig

    IF Staining of A549 using 67776-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CLIP-115/CLIP2 antibody (67776-1-Ig, Clone: 2E6B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inmouse cerebellum tissue, rabbit brain tissue, pig brain tissue, rat brain tissue, mouse brain tissue, pig cerebellum tissue, rat cerebellum tissue, A549 cells
Positive IF detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy CLIP2 Ready-To-Use IHC Kit. CLIP2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:20000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67776-1-Ig targets CLIP-115/CLIP2 in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat, pig, rabbit samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, mouse, rat, pig, rabbit
Immunogen CLIP-115/CLIP2 fusion protein Ag18902 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name CAP-GLY domain containing linker protein 2
Synonyms CLIP, CLIP 115, CLIP-115/CLIP2, CLIP2, CYLN2, Cytoplasmic linker protein 115, Cytoplasmic linker protein 2, KIAA0291, WBSCR3, WBSCR4, WSCR3, WSCR4
Calculated Molecular Weight 1011 aa, 112 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC150332
Gene Symbol CLIP2
Gene ID (NCBI) 7461
RRIDAB_2918541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9UDT6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CILP-2/CLIP-115 is a cytoplasmic linker protein (CLIP) that links membranous organelles to microtubules. CLIP-115 is enriched in the axonal growth cone and it enables neuronal polarization by controlling the stabilization of microtubules. Mutation of CLIP-115 has been associated with Williams-Beuren syndrome.

实验方案

Product Specific Protocols
WB protocol for CLIP-115/CLIP2 antibody 67776-1-IgDownload protocol
IF protocol for CLIP-115/CLIP2 antibody 67776-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols