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验证数据展示

  • WB analysis of K-562 using 83730-3-RR

    WB analysis of K-562 using 83730-3-RR

    K-562 cells were subjected to SDS PAGE followed by western blot with 83730-3-RR (CLIC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 83730-3-RR

    WB analysis using 83730-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83730-3-RR (CLIC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83730-3-RR

    Affinity and Kinetic Characterization of 83730-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83730-3-RR against Human CLIC2 were performed. The affinity constant is below 1 pM.

CLIC2 Recombinant antibody

CLIC2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83730-3-RR

产品说明书

CloneNo. 240726D1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

XAP121, 240726D1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman testis tissue, K-562 cells, human heart tissue, human placenta tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83730-3-RR targets CLIC2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 CLIC2 fusion protein Ag6766 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chloride intracellular channel 2
别名 XAP121, 240726D1
计算分子量 28 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC005367
基因名称 CLIC2
Gene ID (NCBI) 1193
RRIDAB_3671329
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO15247
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CLIC2 (chloride intracellular channel 2), also known as CLCNL2. It is expected to be located in cytoplasm and membrane. CLIC-2 exists as a monomer. It also has an unusual enzyme activity profile. While the CXXC active site motif is conserved between CLIC-2 and the glutaredoxins, no thiol transferase activity was detected. In contrast, low glutathione peroxidase activity was recorded. CLIC-2 was found to be widely distributed in tissues including heart and skeletal muscle. Functional studies showed that CLIC-2 inhibited cardiac ryanodine receptor Ca2+ release channels in lipid bilayers when added to the cytoplasmic side of the channels and inhibited Ca2+ release from cardiac sarcoplasmic reticulum vesicles. The calculated molecular weight of CLIC2 is 28 kDa.

实验方案

Product Specific Protocols
WB protocol for CLIC2 antibody 83730-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols