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Dectin-1 Polyclonal antibody

Dectin-1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 22809-1-AP

Print datasheet

Synonyms

Beta glucan receptor, BGR, CD369, CLEC7A, CLECSF12, DC associated C type lectin 1, Dectin 1, DECTIN1, Dectin-1

验证数据展示

  • WB analysis using 22809-1-AP

    WB analysis using 22809-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22809-1-AP (Dectin-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHL-60 cells, THP-1 cells, U-937 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

22809-1-AP targets Dectin-1 in WB, ELISA applications and shows reactivity with human samples.

Tested Applications WB, ELISA
Tested Reactivity human
Immunogen Dectin-1 fusion protein Ag18924 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name C-type lectin domain family 7, member A
Synonyms Beta glucan receptor, BGR, CD369, CLEC7A, CLECSF12, DC associated C type lectin 1, Dectin 1, DECTIN1, Dectin-1
Calculated Molecular Weight 247 aa, 28 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC013385
Gene Symbol CLEC7A
Gene ID (NCBI) 64581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BXN2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dectin-1, also known as CLEC7A or CD369, is a type II membrane receptor consisting of an extracellular C-terminal C-type lectin domain, a short stalk region, a single transmembrane domain and a short N-terminal cytoplasmic tail, which contains an immunoreceptor tyrosine-based activation motif (PMID: 10779524; 21612412). Dectin-1 is expressed on monocytes, macrophages, neutrophils, dendritic cells and a subpopulation of T cells (PMID: 12244185). Dectin-1 plays a role in the innate immune response. It functions as a pattern-recognition receptor and recognizes a variety of β-glucans from fungi, plants and bacteria. Dectin-1 may also participate in orchestrating the adaptive immune response (PMID: 21612412). The apparent molecular weight is larger than the calculated molecular weight due to glycosylation (PMID: 10779524).

实验方案

Product Specific Protocols
WB protocol for Dectin-1 antibody 22809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols