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验证数据展示

  • WB analysis using 18248-1-AP

    WB analysis using 18248-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18248-1-AP (CLEC5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

CLEC5A Polyclonal antibody

CLEC5A Polyclonal Antibody for WB, ELISA
Cat No. 18248-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

MDL-1, MDL1, MDL 1, C-type lectin superfamily member 5, C-type lectin domain family 5 member A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJ774A.1 cells, K-562 cells, THP-1 cells, RAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18248-1-AP targets CLEC5A in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 CLEC5A fusion protein Ag13025 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 C-type lectin domain family 5, member A
别名 MDL-1, MDL1, MDL 1, C-type lectin superfamily member 5, C-type lectin domain family 5 member A
计算分子量 188 aa, 22 kDa
观测分子量36 kDa
GenBank蛋白编号BC112099
基因名称 CLEC5A
Gene ID (NCBI) 23601
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NY25
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-type lectin domain family 5 member A (CLEC5A, also known as CLECSF5 and MDL1) is a spleen tyrosine kinase (Syk)-coupled receptor abundantly expressed by monocytes, macrophages and neutrophils (PMID: 10449773). It has a pivotal function in activating multiple aspects of innate immunity against bacterial invasion (PMID: 28824166). Both in vitro and in vivo evidence supported that CLEC5A was involved in glioblastoma pathogenesis via regulation of PI3K/Akt pathway (PMID: 30834619). It appeared as a protein band of ~36 kDa because of glycosylation, while its predicted molecular weight is 22 kDa (PMID: 25877931; 19074552).

实验方案

Product Specific Protocols
WB protocol for CLEC5A antibody 18248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols