验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| IHC | See 1 publications below |
产品信息
16196-1-AP targets Claudin 9-specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | claudin 9 |
| 别名 | claudin 9, CLDN9 |
| 计算分子量 | 23 kDa |
| 观测分子量 | 25-29 kDa |
| GenBank蛋白编号 | NM_020982 |
| 基因名称 | Claudin 9 |
| Gene ID (NCBI) | 9080 |
| RRID | AB_2083123 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95484 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 9.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Claudin 9-specific antibody 16196-1-AP | Download protocol |
| IP protocol for Claudin 9-specific antibody 16196-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Histochem Cell Biol Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier. | ||
Front Genet Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma. |