验证数据展示
经过测试的应用
| Positive WB detected in | mouse cerebellum tissue, mouse brain tissue, mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
87798-1-RR targets Claudin 25 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | claudin-like |
| 别名 | Claudin-25, CLDN25, Putative claudin-25 |
| 计算分子量 | 229aa, 25 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | M_001101389 |
| 基因名称 | hCG_1646420 |
| Gene ID (NCBI) | 644672 |
| RRID | AB_3745756 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | C9JDP6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Claudin-25, also known as Claudin containing domain 1, is a tight junction protein. It plays a role in epithelial cell barrier function and ion transport regulation. Predominantly expressed in the blood-brain barrier (BBB) and myelin sheaths, it regulates paracellular permeability. It plays vital roles in maintaining neural homeostasis, and its dysregulation is linked to demyelinating diseases and certain cancers.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Claudin 25 antibody 87798-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
