验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, mouse small intestine tissue, rat colon tissue, rat small intestine tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16302-1-AP targets Claudin 15 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | Claudin 15 fusion protein Ag9124 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | claudin 15 |
| 别名 | CLDN15, Claudin-15 |
| 计算分子量 | 128aa,14 kDa; 228aa,24 kDa |
| 观测分子量 | 24-26 kDa |
| GenBank蛋白编号 | BC010160 |
| 基因名称 | Claudin 15 |
| Gene ID (NCBI) | 24146 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P56746 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Claudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin family member Claudin-15 is a classic tight junction molecule.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Claudin 15 antibody 16302-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |